BOULE Polyclonal antibody

BOULE Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13720-1-AP

Synonyms

bol, boule like (Drosophila), BOLL, BOULE, Protein boule like



Tested Applications

Positive WB detected inmouse testis tissue
Positive IP detected inmouse testis tissue
Positive IHC detected inmouse ovary tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

13720-1-AP targets BOULE in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BOULE fusion protein Ag4662
Full Name bol, boule-like (Drosophila)
Calculated molecular weight 283 aa, 31 kDa
Observed molecular weight 38-42 kDa
GenBank accession numberBC033674
Gene symbol BOLL
Gene ID (NCBI) 66037
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for BOULE antibody 13720-1-APDownload protocol
IHC protocol for BOULE antibody 13720-1-APDownload protocol
IP protocol for BOULE antibody 13720-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols