CoraLite® Plus 488-conjugated BOULE Polyclonal antibody

BOULE Polyclonal Antibody for IF

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-13720

Synonyms

bol, boule like (Drosophila), BOLL, BOULE, Protein boule like

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Validation Data Gallery

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  IF Staining of mouse testis using CL488-13720

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CoraLite® Plus 488 BOULE antibody (CL488-13720) at dilution of 1:400, CoraLite®594 DAZL antibody (CL594-12633, red), CoraLite® Plus 647 TNP1 antibody (CL647-17178, Magenta).

• 

  IF Staining of mouse testis using CL488-13720

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CoraLite® Plus 488 BOULE antibody (CL488-13720) at dilution of 1:400, CoraLite®594 DAZL antibody (CL594-12633, red), CoraLite® Plus 647 TNP1 antibody (CL647-17178, Magenta).

• 

  IF Staining of mouse testis using CL488-13720

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CoraLite® Plus 488 BOULE antibody (CL488-13720) at dilution of 1:200.

• 

  IF Staining of mouse testis using CL488-13720

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CoraLite® Plus 488 BOULE antibody (CL488-13720) at dilution of 1:200.

• 

  IF Staining of mouse testis using CL488-13720

  Immunofluorescent analysis of (cold ethanol) fixed mouse testis tissue using CoraLite® Plus 488 BOULE antibody (CL488-13720) at dilution of 1:100, CoraLite®555 TNP1 antibody (CL555-17178, orange), STAR antibody (67130-1-Ig, Clone: 1G6E6, Magenta).

• 

  IF Staining of mouse testis using CL488-13720

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CoraLite® Plus 488 BOULE antibody (CL488-13720) at dilution of 1:200, CoraLite®594 PRM1 antibody (CL594-15697, red).

• 

  IF Staining of mouse testis using CL488-13720

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CoraLite® Plus 488 BOULE antibody (CL488-13720) at dilution of 1:200, CoraLite®594 PRM1 antibody (CL594-15697, red).

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Tested Applications

• Positive IF detected in: mouse testis tissue

Recommended dilution

• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-13720 targets BOULE in IF applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BOULE fusion protein Ag4662
• Full Name: bol, boule-like (Drosophila)
• Calculated Molecular Weight: 283 aa, 31 kDa
• GenBank Accession Number: BC033674
• Gene Symbol: BOLL
• Gene ID (NCBI): 66037
• RRID: AB_2919076
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BOULE, also called BOLL, belongs to the RRM DAZ family. BOULE has been identified as being involved in regulating the development and differentiation of germ cells and playing an essential role in infertility (PMID: 31683986). BOULE is specifically expressed in testis, but not in early embryos, primordial germ cells, and spermatogonial cells. It is first expressed in the cytoplasm of spermatocytes and then persists through meiosis. BOULE has 5 isoforms with the molecular mass of 19 and 31-38 kDa. This antibody detects two bands in testis, the lower band of ~40 kDa in size corresponds to the predicted endogenous BOULE protein, while the upper band may be an isoform or posttranslationally modified version of the BOULE protein (PMID: 27632217).