Beta galactosidase Antibody

Beta galactosidase Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

4F4F4

Cat no : 66586-1-Ig

Synonyms

Acid beta galactosidase, Beta galactosidase, EBP, Elastin receptor 1, ELNR1, galactosidase, beta 1, GLB1, Lactase



Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, BxPC-3 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:150-1:600
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

66586-1-Ig targets Beta galactosidase in WB, IHC, IF, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Beta galactosidase fusion protein Ag8069
Full Name galactosidase, beta 1
Calculated molecular weight 76 kDa
Observed molecular weight 64-66 kDa, 76-85 kDa
GenBank accession number BC007493
Gene symbol GLB1
Gene ID (NCBI) 2720
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GLB1(Beta-galactosidase) is also named as ELNR1 or Lactase. It cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans. This protein is identical to the elastin-binding protein (EBP), a major component of the nonintegrin cell surface receptor complex expressed in fibroblasts, smooth muscle cells, chondroblasts, leukocytes, and certain cancer cell types. Defects in GLB1 are the cause of GM1-gangliosidosis type 1 (GM1G1), GM1-gangliosidosis type 2 (GM1G2), GM1-gangliosidosis type 3 (GM1G3) and mucopolysaccharidosis type 4B (MPS4B). GBL1 is synthesized as an 85-kDa precursor that is C-terminally processed into a 64-66 kDa mature form and the released ~20-kDa proteolytic fragment was thought to be degraded (PMID: 10744681). GLB1 has 3 isoforms with MW of 76 kDa, 73 kda and 61 kDa.

Protocols

Product Specific Protocols
WB protocol for Beta galactosidase antibody 66586-1-IgDownload protocol
IHC protocol for Beta galactosidase antibody 66586-1-IgDownload protocol
IF protocol for Beta galactosidase antibody 66586-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols