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  • KD/KO Validated

MYDGF/C19orf10 Polyclonal antibody

MYDGF/C19orf10 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11353-1-AP

Synonyms

C19orf10, MYDGF, Myeloid-derived growth factor, SF20



Tested Applications

Positive WB detected inDU 145 cells, HeLa cells, mouse colon tissue, Jurkat cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11353-1-AP targets MYDGF/C19orf10 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MYDGF/C19orf10 fusion protein Ag1900
Full Name chromosome 19 open reading frame 10
Calculated Molecular Weight 173 aa, 19 kDa
Observed Molecular Weight 15-19 kDa
GenBank Accession NumberBC010129
Gene Symbol C19orf10
Gene ID (NCBI) 56005
RRIDAB_2207647
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Myeloid-derived growth factor (MYDGF), also named as chromosome 19 open reading frame 10 (C19orf10), is an FLS-derived protein that is secreted into the synovial fluid. MYDGF as a paracrine-acting protein secreted from monocytes/macrophages that improves tissue repair and heart function after myocardial infarction. MYDGF has been reported to regulates neutrophil motility in interstitial tissue damage(PMID: 34047769).

Protocols

Product Specific Protocols
WB protocol for MYDGF/C19orf10 antibody 11353-1-APDownload protocol
IHC protocol for MYDGF/C19orf10 antibody 11353-1-APDownload protocol
IF protocol for MYDGF/C19orf10 antibody 11353-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IF

Sci Adv

Myeloid-derived growth factor inhibits inflammation and alleviates endothelial injury and atherosclerosis in mice.

Authors - Biying Meng
  • KO Validated
human,mouse,ratWB,IHC,IF

Kidney Int

Myeloid-derived Growth Factor Deficiency Exacerbates Mitotic Catastrophe of Podocytes in Glomerular Disease.

Authors - Ping Zhan
  • KO Validated
humanWB,IHC

Front Oncol

The Clinical Relevance and Tumor Promoting Function of C19orf10 in Kidney Renal Clear Cell Carcinoma.

Authors - Yanxin Lu
  • KD Validated
humanIHC,WB

J Cancer

C19orf10 promotes malignant behaviors of human bladder carcinoma cells via regulating the PI3K/AKT and Wnt/β-catenin pathways.

Authors - Shi Li
  • KD Validated
human,mouseWB,IHC,IF

Cell Death Dis

Myeloid-derived growth factor alleviates non-alcoholic fatty liver disease alleviates in a manner involving IKKβ/NF-κB signaling

Authors - Yan Ding
humanWB

Nat Commun

Identification of myeloid-derived growth factor as a mechanically-induced, growth-promoting angiocrine signal for human hepatocytes

Authors - Linda Große-Segerath