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C1D Polyclonal antibody

C1D Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10711-1-AP

Synonyms

C1D, hC1D, LRP1, SUN CoR, SUNCOR

Validation Data Gallery

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  • Western Blot (WB) analysis of DU 145 cells using C1D Polyclonal antibody (10711-1-AP)

    WB analysis of DU 145 using 10711-1-AP

    DU 145 cells were subjected to SDS PAGE followed by western blot with 10711-1-AP (C1D Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of DU 145 cells using C1D Polyclonal antibody (10711-1-AP)

    IP experiment of DU 145 using 10711-1-AP

    IP result of anti-C1D (IP:10711-1-AP, 4ug; Detection:10711-1-AP 1:300) with DU 145 cells lysate 1800 ug.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using C1D Polyclonal antibody (10711-1-AP)

    IHC staining of human prostate cancer using 10711-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10711-1-AP (C1D Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using C1D Polyclonal antibody (10711-1-AP)

    IHC staining of human prostate cancer using 10711-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10711-1-AP (C1D Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using C1D Polyclonal antibody (10711-1-AP)

    IHC staining of human prostate cancer using 10711-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10711-1-AP (C1D Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using C1D Polyclonal antibody (10711-1-AP)

    IHC staining of human prostate cancer using 10711-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10711-1-AP (C1D Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using C1D Polyclonal antibody (10711-1-AP)

    IHC staining of human prostate cancer using 10711-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10711-1-AP (C1D antibody) at dilution of 1:100 (under 10x lens).

Tested Applications

Positive WB detected inDU 145 cells
Positive IP detected inDU 145 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

10711-1-AP targets C1D in WB, IP, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C1D fusion protein Ag1121
Full Name C1D nuclear receptor co-repressor
Calculated Molecular Weight 16 kDa
GenBank Accession NumberBC005235
Gene Symbol C1D
Gene ID (NCBI) 10438
RRIDAB_2067014
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for C1D antibody 10711-1-APDownload protocol
IHC protocol for C1D antibody 10711-1-APDownload protocol
IP protocol for C1D antibody 10711-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Redox Biol

Multi-omics profiling reveals Chitinase-3-like protein 1 as a key mediator in the crosstalk between sarcopenia and liver cancer

Authors - Di Lu
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Ralf (Verified Customer) (08-04-2022)

10µg of total mouse protein were loaded on the gel. Antibodies also stain the ladder.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:200
C1D Antibody Western Blot validation (1:200 dilution) in (Cat no:10711-1-AP)