Tested Applications
| Positive WB detected in | mouse spleen tissue, mouse lung tissue |
| Positive IHC detected in | human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
85719-4-RR targets C1QA in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Eg2396 Product name: Recombinant Human C1QA protein (rFc Tag) Source: mammalian cells-derived, pHZ-KIsec-N-rFc Tag: N-rFc Domain: 23-245 aa of NM_015991.4 Sequence: EDLCRAPDGKKGEAGRPGRRGRPGLKGEQGEPGAPGIRTGIQGLKGDQGEPGPSGNPGKVGYPGPSGPLGARGIPGIKGTKGSPGNIKDQPRPAFSAIRRNPPMGGNVVIFDTVITNQEEPYQNHSGRFVCTVPGYYYFTFQVLSQWEICLSIVSSSRGQVRRSLGFCDTTNKGLFQVVSGGMVLQLQQGDQVWVEKDPKKGHIYQGSEADSVFSGFLIFPSA Predict reactive species |
| Full Name | complement component 1, q subcomponent, A chain |
| Calculated Molecular Weight | 26kDa |
| Observed Molecular Weight | 26-30 kDa |
| GenBank Accession Number | NM_015991.4 |
| Gene Symbol | C1QA |
| Gene ID (NCBI) | 712 |
| RRID | AB_3744317 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P02745 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The first component of complement, C1, is a calcium-dependent complex of the 3 subcomponents C1q, C1r, and C1s. C1q is composed of 18 polypeptide chains: six A-chains, six B-chains, and six C-chains. Each chain contains a collagen-like region located near the N terminus and a C-terminal globular region. Deficiency of C1q has been associated with lupus erythematosus and glomerulonephritis. This antibody is raised against C1qA which is the A-chain polypeptide of human complement subcomponent C1q.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for C1QA antibody 85719-4-RR | Download protocol |
| IHC protocol for C1QA antibody 85719-4-RR | Download protocol |
| WB protocol for C1QA antibody 85719-4-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The C1QA Recombinant monoclonal antibody (250093H1), catalog number 85719-4-RR, has 1 total citation. The cited publication appeared in Investigative Ophthalmology & Visual Science (Impact Factor 5.5), published in January 2026. The study focused on early transcriptomic and pathologic changes in Col8a2 mutant Fuchs endothelial corneal dystrophy, placing the research in the fields of ophthalmology and corneal disease. The antibody was used for immunofluorescence (IF) on mouse samples.
| Species | Application | Title |
|---|---|---|
Invest Ophthalmol Vis Sci Early Transcriptomic and Pathologic Changes of Col8a2 Mutant Fuchs Endothelial Corneal Dystrophy. |
