|Positive WB detected in||HL-60 cells, NIH/3T3 cells, mouse brain tissue, HeLa cells, rat brain tissue, COLO 320 cells, human skeletal muscle tissue, HepG2 cells, MCF-7 cells, SH-SY5Y cells|
|Positive IHC detected in||human thyroid tissue, human skeletal muscle tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
10292-1-AP targets calreticulin in WB, IHC,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||calreticulin fusion protein Ag0325|
|Calculated molecular weight||60 kDa|
|Observed molecular weight||55 kDa|
|GenBank accession number||BC002500|
|Gene ID (NCBI)||811|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
CALR,also named as grp60, ERp60, HACBP, CRP55, CRTC and Calregulin, belongs to the calreticulin family. It is a molecular calcium-binding chaperone promoting folding, oligomeric assembly and quality control in the ER via the calreticulin/calnexin cycle. CALR is a ER marker. It interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. CALR interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export. The MW of CALR migrates aberrantly at 55 kDa by SDS-PAGE. Some study provided that it's a new possibility for CRT-mediated tumor immune prevention and treatment.
J Extracell Vesicles
Raman spectroscopy as a quick tool to assess purity of extracellular vesicle preparations and predict their functionality.
Eur J Med Chem
Naphthyl quinoxaline thymidine conjugate is a potent anticancer agent post UVA activation and elicits marked inhibition of tumor growth through vaccination.
Combination of CALR and PDIA3 is a potential prognostic biomarker for non-small cell lung cancer.
Myc-mediated SDHA acetylation triggers epigenetic regulation of gene expression and tumorigenesis.
Aging (Albany NY)
Calreticulin: a potential diagnostic and therapeutic biomarker in gallbladder cancer.
Proteomic analysis of retinal pigment epithelium cells after exposure to UVA radiation.