• Featured Product
  • KD/KO Validated

calreticulin Antibody

calreticulin Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10292-1-AP

Synonyms

CALR, Calregulin, calreticulin, cC1qR, CRP55, CRT, CRTC, ERp60, grp60, HACBP, RO, SSA



Tested Applications

Positive WB detected inHL-60 cells, mouse brain tissue, rat brain tissue, HeLa cells, human skeletal muscle tissue, COLO 320 cells, NIH/3T3 cells, HeLa cells, HepG2 cells, MCF-7 cells, SH-SY5Y cells, MDA-MB-231 cells
Positive IHC detected inhuman thyroid tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10292-1-AP targets calreticulin in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen calreticulin fusion protein Ag0325
Full Name calreticulin
Calculated molecular weight 60 kDa
Observed molecular weight 55 kDa
GenBank accession number BC002500
Gene symbol CALR
Gene ID (NCBI) 811
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CALR,also named as grp60, ERp60, HACBP, CRP55, CRTC and Calregulin, belongs to the calreticulin family. It is a molecular calcium-binding chaperone promoting folding, oligomeric assembly and quality control in the ER via the calreticulin/calnexin cycle. CALR is a ER marker. It interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. CALR interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export. The MW of CALR migrates aberrantly at 55 kDa by SDS-PAGE. Some study provided that it's a new possibility for CRT-mediated tumor immune prevention and treatment.

Protocols

Product Specific Protocols
WB protocol for calreticulin antibody 10292-1-APDownload protocol
IHC protocol for calreticulin antibody 10292-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Extracell Vesicles

Raman spectroscopy as a quick tool to assess purity of extracellular vesicle preparations and predict their functionality.

Authors - Alice Gualerzi
ratIF

Eur J Med Chem

Naphthyl quinoxaline thymidine conjugate is a potent anticancer agent post UVA activation and elicits marked inhibition of tumor growth through vaccination.

Authors - Ying Yuan
humanIF

Oncotarget

Combination of CALR and PDIA3 is a potential prognostic biomarker for non-small cell lung cancer.

Authors - Ke Wang
humanWB

BMC Ophthalmol

Proteomic analysis of retinal pigment epithelium cells after exposure to UVA radiation.

Authors - Jiunn-Liang Chen
IHC

Onco Targets Ther

Identification of PGAM1 as a putative therapeutic target for pancreatic ductal adenocarcinoma metastasis using quantitative proteomics.

Authors - Xinlu Liu
humanIHC

Diagn Pathol

A comparative proteomic study identified calreticulin and prohibitin up-regulated in adrenocortical carcinomas.

Authors - Yang Ming-shan MS