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CAP2 Polyclonal antibody

Name: CAP2 Polyclonal antibody
Brand: Proteintech
SKU: 15865-1-AP
Rating: 5.00 (1 reviews)

CAP2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Publications

Reviews (1)

Cat no : 15865-1-AP

Synonyms

Adenylyl cyclase associated protein 2, CAP 2, CAP2

Validation Data Gallery

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AllWBIPIHCIF

WB analysis of human kidney using 15865-1-AP
human kidney tissue were subjected to SDS PAGE followed by western blot with 15865-1-AP (CAP2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis using 15865-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15865-1-AP (CAP2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of mouse heart using 15865-1-AP
mouse heart tissue were subjected to SDS PAGE followed by western blot with 15865-1-AP (CAP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 15865-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 15865-1-AP (CAP2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of rat testis using 15865-1-AP
rat testis tissue were subjected to SDS PAGE followed by western blot with 15865-1-AP (CAP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of mouse skeletal muscle using 15865-1-AP
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15865-1-AP (CAP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse testis using 15865-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 15865-1-AP (CAP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of PC-3 using 15865-1-AP
PC-3 cells were subjected to SDS PAGE followed by western blot with 15865-1-AP (CAP2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of mouse kidney using 15865-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15865-1-AP (CAP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IP experiment of mouse testis using 15865-1-AP
IP result of anti-CAP2 (IP:15865-1-AP, 4ug; Detection:15865-1-AP 1:1000) with mouse testis tissue lysate 4000ug.
IHC staining of human liver cancer using 15865-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15865-1-AP (CAP2 Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human liver cancer using 15865-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15865-1-AP (CAP2 Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human skeletal muscle using 15865-1-AP
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 15865-1-AP (CAP2 Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human skeletal muscle using 15865-1-AP
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 15865-1-AP (CAP2 Antibody) at dilution of 1:50 (under 40x lens).
IF Staining of U2OS using 15865-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using 15865-1-AP (CAP2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of U2OS using 15865-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using CAP2 antibody (15865-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Cat No. 15865-1-AP

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Tested Applications

Positive WB detected in	PC-3 cells, mouse skeletal muscle tissue, mouse testis tissue, mouse heart tissue, HepG2 cells, mouse kidney tissue, rat testis tissue, human kidney tissue
Positive IP detected in	mouse testis tissue
Positive IHC detected in	human liver cancer tissue, human skeletal muscle tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF detected in	U2OS cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)	IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 4 publications below
IHC	See 1 publications below
IF	See 2 publications below
CoIP	See 1 publications below

Product Information

15865-1-AP targets CAP2 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CAP2 fusion protein Ag8477
Full Name	CAP, adenylate cyclase-associated protein, 2 (yeast)
Calculated Molecular Weight	477 aa, 53 kDa
Observed Molecular Weight	53 kDa
GenBank Accession Number	BC008481
Gene Symbol	CAP2
Gene ID (NCBI)	10486
RRID	AB_2270174
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CAPs (CAP1 and CAP2) are evolutionarily conserved proteins with roles in regulating the actin cytoskeleton and in signal transduction. CAP2 is predominantly expressed in brain, heart and skeletal muscle, and skin. It is found in the nucleus in undifferentiated myoblasts and at the M-line of differentiated myotubes. Overexpression of CAP2 has been reported in many cancers, including hepatocellular carcinoma (HCC), human breast cancer, and malignant melanoma.

Protocols

Product Specific Protocols
WB protocol for CAP2 antibody 15865-1-AP	Download protocol
IHC protocol for CAP2 antibody 15865-1-AP	Download protocol
IF protocol for CAP2 antibody 15865-1-AP	Download protocol
IP protocol for CAP2 antibody 15865-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,IHC,IF,CoIP	J Clin Invest CAP2 promotes gastric cancer metastasis by mediating the interaction between tumor cells and tumor-associated macrophages Authors - Guohao Zhang KD Validated View Article
human	WB	Int J Mol Sci Analysis of mRNA and Protein Levels of CAP2, DLG1 and ADAM10 Genes in Post-Mortem Brain of Schizophrenia, Parkinson's and Alzheimer's Disease Patients. Authors - Anna Di Maio View Article
mouse,rat	WB,IF	Commun Biol CAP2 is a regulator of actin pointed end dynamics and myofibrillogenesis in cardiac muscle. Authors - Mert Colpan KO Validated View Article
mouse	WB	Sci Rep Cyclase-associated protein 2 (CAP2) controls MRTF-A localization and SRF activity in mouse embryonic fibroblasts. Authors - Lara-Jane Kepser View Article
		Oncol Rep Expression status of cyclase‑associated protein 2 as a prognostic marker for human breast cancer. Authors - Lihua Xu View Article

Reviews

Add a review

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

Anil K (Verified Customer) (06-10-2022)

Saw a single band in the mice brain lysates

Applications: Western Blot
Cell Tissue Type: brain

CAP2 Polyclonal antibody

CAP2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Validation Data Gallery

WB analysis of human kidney using 15865-1-AP

WB analysis using 15865-1-AP

WB analysis of mouse heart using 15865-1-AP

WB analysis of HepG2 using 15865-1-AP

WB analysis of rat testis using 15865-1-AP

WB analysis of mouse skeletal muscle using 15865-1-AP

WB analysis of mouse testis using 15865-1-AP

WB analysis of PC-3 using 15865-1-AP

WB analysis of mouse kidney using 15865-1-AP

IP experiment of mouse testis using 15865-1-AP

IHC staining of human liver cancer using 15865-1-AP

IHC staining of human liver cancer using 15865-1-AP

IHC staining of human skeletal muscle using 15865-1-AP

IHC staining of human skeletal muscle using 15865-1-AP

IF Staining of U2OS using 15865-1-AP

IF Staining of U2OS using 15865-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

CAP2 promotes gastric cancer metastasis by mediating the interaction between tumor cells and tumor-associated macrophages

Analysis of mRNA and Protein Levels of CAP2, DLG1 and ADAM10 Genes in Post-Mortem Brain of Schizophrenia, Parkinson's and Alzheimer's Disease Patients.

CAP2 is a regulator of actin pointed end dynamics and myofibrillogenesis in cardiac muscle.

Cyclase-associated protein 2 (CAP2) controls MRTF-A localization and SRF activity in mouse embryonic fibroblasts.

Expression status of cyclase‑associated protein 2 as a prognostic marker for human breast cancer.

Reviews