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CAP2 Polyclonal antibody

CAP2 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 15865-1-AP

Synonyms

Adenylyl cyclase associated protein 2, CAP 2, CAP2

Validation Data Gallery

  • WB analysis of human kidney using 15865-1-AP

    WB analysis of human kidney using 15865-1-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 15865-1-AP (CAP2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IF Staining of U2OS using 15865-1-AP

    IF Staining of U2OS using 15865-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using 15865-1-AP (CAP2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IP experiment of mouse testis using 15865-1-AP

    IP experiment of mouse testis using 15865-1-AP

    IP Result of anti-CAP2 (IP:15865-1-AP, 4ug; Detection:15865-1-AP 1:1000) with mouse testis tissue lysate 4000ug.

  • IHC staining of human liver cancer using 15865-1-AP

    IHC staining of human liver cancer using 15865-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15865-1-AP (CAP2 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human liver cancer using 15865-1-AP

    IHC staining of human liver cancer using 15865-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15865-1-AP (CAP2 Antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inhuman kidney tissue, HepG2 cells, mouse heart tissue, mouse kidney tissue, mouse skeletal muscle tissue, mouse testis tissue, PC-3 cells, rat testis tissue
Positive IP detected inmouse testis tissue
Positive IHC detected inhuman liver cancer tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

15865-1-AP targets CAP2 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CAP2 fusion protein Ag8477
Full Name CAP, adenylate cyclase-associated protein, 2 (yeast)
Calculated molecular weight 477 aa, 53 kDa
Observed molecular weight 53 kDa
GenBank accession numberBC008481
Gene symbol CAP2
Gene ID (NCBI) 10486
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for CAP2 antibody 15865-1-APDownload protocol
IHC protocol for CAP2 antibody 15865-1-APDownload protocol
IF protocol for CAP2 antibody 15865-1-APDownload protocol
IP protocol for CAP2 antibody 15865-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Oncol Rep

Expression status of cyclase‑associated protein 2 as a prognostic marker for human breast cancer.

Authors - Lihua Xu
View Article
mouse,ratWB,IF

Commun Biol

CAP2 is a regulator of actin pointed end dynamics and myofibrillogenesis in cardiac muscle.

Authors - Mert Colpan
  • KO Validated
View Article