CAP2 Polyclonal antibody
CAP2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 15865-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | human kidney tissue, HepG2 cells, mouse heart tissue, mouse kidney tissue, mouse skeletal muscle tissue, mouse testis tissue, PC-3 cells, rat testis tissue |
Positive IP detected in | mouse testis tissue |
Positive IHC detected in | human liver cancer tissue, human skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | U2OS cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:3000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Product Information
15865-1-AP targets CAP2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | CAP2 fusion protein Ag8477 |
Full Name | CAP, adenylate cyclase-associated protein, 2 (yeast) |
Calculated molecular weight | 477 aa, 53 kDa |
Observed molecular weight | 53 kDa |
GenBank accession number | BC008481 |
Gene symbol | CAP2 |
Gene ID (NCBI) | 10486 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
Product Specific Protocols | |
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WB protocol for CAP2 antibody 15865-1-AP | Download protocol |
IHC protocol for CAP2 antibody 15865-1-AP | Download protocol |
IF protocol for CAP2 antibody 15865-1-AP | Download protocol |
IP protocol for CAP2 antibody 15865-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Oncol Rep Expression status of cyclase‑associated protein 2 as a prognostic marker for human breast cancer. |