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  • KD/KO Validated

Calpain 1 Polyclonal antibody

Calpain 1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 10538-1-AP

Synonyms

Calpain 1, Calpain 1 catalytic subunit, Calpain 1 large subunit, Calpain mu type, CANP, CANP 1, CANP1, CANPL1, CAPN1, Micromolar calpain, muCANP, muCL



Tested Applications

Positive WB detected inHeLa cells, mouse testis tissue, A2780 cells, A431 cells, HepG2 cells, PC-3 cells, K-562 cells, Jurkat cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman urothelial carcinoma tissue, human heart tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

10538-1-AP targets Calpain 1 in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Calpain 1 fusion protein Ag0810
Full Name calpain 1, (mu/I) large subunit
Calculated molecular weight 704 aa, 82 kDa
Observed molecular weight 75 kDa
GenBank accession numberBC008751
Gene symbol CAPN1
Gene ID (NCBI) 823
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for Calpain 1 antibody 10538-1-APDownload protocol
IHC protocol for Calpain 1 antibody 10538-1-APDownload protocol
IF protocol for Calpain 1 antibody 10538-1-APDownload protocol
IP protocol for Calpain 1 antibody 10538-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Pharmacol Res

Utility of 7,8-dihydroxyflavone in preventing astrocytic and synaptic deficits in the hippocampus elicited by PTSD.

Authors - Juan Wang
mouseWB

Pharmacol Res

TRPC1 contributes to endotoxemia-induced myocardial dysfunction via mediating myocardial apoptosis and autophagy.

Authors - Wen Tian
human,mouseWB

Biochim Biophys Acta Mol Cell Res

Elastic titin properties and protein quality control in the aging heart.

Authors - Senem Salcan
mouseWB

Biochim Biophys Acta Mol Cell Biol Lipids

Over-activation of NMDA receptors promotes ABCA1 degradation and foam cell formation.

Authors - Haipeng Cheng
mouse,ratWB,IF

J Cell Mol Med

Calpain-1 mediates vascular remodelling and fibrosis via HIF-1α in hypoxia-induced pulmonary hypertension.

Authors - Haiyan Deng
mouseIF,WB

Front Pharmacol

Protective effect of Astragaloside IV on chronic intermittent hypoxia-induced vascular endothelial dysfunction through the calpain-1/SIRT1/AMPK signaling pathway

Authors - Fang Zhao

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

James (Verified Customer) (03-31-2021)

Antibody detected endogenous and overexpressed Calpain-1 in HEK cells

  • Applications: Western Blot, Cell culture
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HEK cell overexpression
FH

Wei (Verified Customer) (03-12-2020)

Single, strong western blot band at predicted size.

  • Applications: Western Blot,
  • Cell Tissue Type: Mouse Heart