|Positive WB detected in||HeLa cells, SH-SY5Y cells, RAW 264.7 cells, MCF-7 cells, NIH/3T3 cells, A549 cells, A431 cells, HepG2 cells, SW 1990 cells|
|Positive FC detected in||SH-SY5Y cells|
|Western Blot (WB)||WB : 1:5000-1:20000|
|Sample-dependent, check data in validation data gallery|
60186-1-Ig targets Cyclin D1 in WB, IF, FC,ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||bovine, hamster, human, mouse, zebrafish|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||Cyclin D1 fusion protein Ag0689|
|Full Name||cyclin D1|
|Calculated molecular weight||295 aa, 34 kDa|
|Observed molecular weight||34 kDa|
|GenBank accession number||BC000076|
|Gene ID (NCBI)||595|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
CCND1 (cyclin D1), also known as PRAD1 or BCL1, belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. CCND1 forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. The CCND1 gene, located on 11q13 has been reported to be overexpressed in mantle cell lymphoma (MCL) due to the chromosomal translocation. CCND1 has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Over-expression of CCND1 is known to correlate with the early onset of cancer and risk of tumor progression and metastasis.
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Janardhan (Verified Customer) (08-22-2020)
Antibody works ok, needs longer exposure
Yuan (Verified Customer) (01-23-2020)
The antibody is ok. Signal is not very strong for western blot. Needed relative long exposure for Hela cells.
Kyosuke (Verified Customer) (06-12-2019)
I use this as a proliferation marker and this antibody works very well for WB.