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Cyclin D1 Monoclonal antibody
Cyclin D1 Monoclonal Antibody for FC, WB, ELISA
Cat no : 60186-1-Ig
Validation Data Gallery
|Positive WB detected in||HCT 116 cells, SH-SY5Y cells, RAW 264.7 cells, HeLa cells, A549 cells, MCF-7 cells, NIH/3T3 cells, A431 cells, HepG2 cells, SW 1990 cells, HSC-T6 cells, PC-12 cells|
|Positive FC detected in||SH-SY5Y cells|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Sample-dependent, check data in validation data gallery|
60186-1-Ig targets Cyclin D1 in WB, IP, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, bovine, hamster, zebrafish|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||Cyclin D1 fusion protein Ag0689|
|Full Name||cyclin D1|
|Calculated molecular weight||295 aa, 34 kDa|
|Observed molecular weight||34 kDa|
|GenBank accession number||BC000076|
|Gene ID (NCBI)||595|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
CCND1 (cyclin D1), also known as PRAD1 or BCL1, belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. CCND1 forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. The CCND1 gene, located on 11q13 has been reported to be overexpressed in mantle cell lymphoma (MCL) due to the chromosomal translocation. CCND1 has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Over-expression of CCND1 is known to correlate with the early onset of cancer and risk of tumor progression and metastasis.
Nat Cell Biol
TRIM15 and CYLD regulate ERK activation via lysine-63-linked polyubiquitination.
YTHDF1 links hypoxia adaptation and non-small cell lung cancer progression.
J Clin Invest
Circular RNA circEsyt2 regulates vascular smooth muscle cell remodeling via splicing regulation.
J Pineal Res
Mitochondrial cytochrome P450 (CYP) 1B1 is responsible for melatonin-induced apoptosis in neural cancer cells.
TSPAN1 promotes autophagy flux and mediates cooperation between WNT-CTNNB1 signaling and autophagy via the MIR454-FAM83A-TSPAN1 axis in pancreatic cancer.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Janardhan (Verified Customer) (08-22-2020)
Antibody works ok, needs longer exposure
Yuan (Verified Customer) (01-23-2020)
The antibody is ok. Signal is not very strong for western blot. Needed relative long exposure for Hela cells.
Kyosuke (Verified Customer) (06-12-2019)
I use this as a proliferation marker and this antibody works very well for WB.