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Cyclin D1 Antibody

Cyclin D1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse and More (3)

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

298

CloneNo.

2G3G5

Cat no : 60186-1-Ig

Synonyms

B cell lymphoma 1 protein, CCND1, cyclin D1, D11S287E, G1/S specific cyclin D1, PRAD1, PRAD1 oncogene, U21B31



Tested Applications

Positive WB detected inHeLa cells, SH-SY5Y cells, RAW 264.7 cells, MCF-7 cells, NIH/3T3 cells, A549 cells, A431 cells, HepG2 cells, SW 1990 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:20000
Sample-dependent, check data in validation data gallery

Product Information

60186-1-Ig targets Cyclin D1 in WB, ELISA applications and shows reactivity with human, mouse samples.

Reactivity human, mouse
Cited Species cow, hamster, human, mouse, zebrafish
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Cyclin D1 fusion protein Ag0689
Full Name cyclin D1
Calculated molecular weight 295aa,34 kDa
Observed molecular weight 34 kDa
GenBank accession number BC000076
Gene symbol CCND1
Gene ID (NCBI) 595
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20oC. Aliquoting is unnecessary for -20oC storage.

Background Information

CCND1 (cyclin D1), also known as PRAD1 or BCL1, belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. CCND1 forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. The CCND1 gene, located on 11q13 has been reported to be overexpressed in mantle cell lymphoma (MCL) due to the chromosomal translocation. CCND1 has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Over-expression of CCND1 is known to correlate with the early onset of cancer and risk of tumor progression and metastasis.

Protocols

Product Specific Protocols
WB protocol for Cyclin D1 antibody 60186-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cancer Sci

Nuclear factor I/B promotes colorectal cancer cell proliferation, epithelial-mesenchymal transition and 5-fluorouracil resistance.

Authors - ZhengYi Liu
humanWB

Biochem Biophys Res Commun

Long non-coding RNA FAM84B-AS promotes resistance of gastric cancer to platinum drugs through inhibition of FAM84B expression.

Authors - Yeqian Zhang
humanWB

Int J Food Sci Nutr

Sulforaphane inhibits gastric cancer stem cells via suppressing sonic hedgehog pathway.

Authors - Miaomiao Ge
humanWB

Oncol Lett

JTC-801 inhibits the proliferation and metastasis of the Hep G2 hepatoblastoma cell line by regulating the phosphatidylinositol 3-kinase/protein kinase B signalling pathway.

Authors - Bufei Zhao
cowWB

J Cell Physiol

NUCKS1 is a novel regulator of milk synthesis in and proliferation of mammary epithelial cells via the mTOR signaling pathway.

Authors - Xiaohan Yuan
mouseWB

Biochem Biophys Res Commun

Poly(ADP-ribose) Polymerase-1 is required for hepatocyte proliferation and liver regeneration in mice.

Authors - Chenhui Ju

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Yuan Xie (01-23-2020)

The antibody is ok. Signal is not very strong for western blot. Needed relative long exposure for Hela cells.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: Hela cell
No image submitted with review
FH

Kyosuke Kazama (06-12-2019)

I use this as a proliferation marker and this antibody works very well for WB.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: rat aortic smooth muscle cells
No image submitted with review