Cyclin H Polyclonal antibody

Cyclin H Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 10718-1-AP

Synonyms

CCNH, p37, p34, MO15-associated protein, MO15 associated protein



Tested Applications

Positive WB detected inA431 cells, A375 cells, mouse brain tissue, HuH-7 cells, HeLa cells, Jurkat cells, K-562 cells, NIH/3T3 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10718-1-AP targets Cyclin H in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cyclin H fusion protein Ag1088
Full Name cyclin H
Calculated Molecular Weight 38 kDa
Observed Molecular Weight 38 kDa
GenBank Accession NumberBC005280
Gene Symbol Cyclin H
Gene ID (NCBI) 902
RRIDAB_2073749
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for Cyclin H antibody 10718-1-APDownload protocol
IHC protocol for Cyclin H antibody 10718-1-APDownload protocol
IF protocol for Cyclin H antibody 10718-1-APDownload protocol
IP protocol for Cyclin H antibody 10718-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols