Various lysates were subjected to SDS PAGE followed by western blot with 10718-1-AP (Cyclin H antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

HuH-7 cells were subjected to SDS PAGE followed by western blot with 10718-1-AP (Cyclin H antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

A375 cells were subjected to SDS PAGE followed by western blot with 10718-1-AP (Cyclin H antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

mouse brain tissue were subjected to SDS PAGE followed by western blot with 10718-1-AP (Cyclin H antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

IP result of anti-Cyclin H (IP:10718-1-AP, 4ug; Detection:10718-1-AP 1:1000) with HeLa cells lysate 1200ug.

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10718-1-AP (Cyclin H antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10718-1-AP (Cyclin H antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (4% PFA) fixed A431 cells using Cyclin H antibody (10718-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

1x10^6 A431 cells were intracellularly stained with 0.25 ug Cyclin H Polyclonal antibody (10718-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).