• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

MME,CD10 Polyclonal antibody

MME,CD10 Polyclonal Antibody for FC, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 23782-1-AP

Synonyms

Atriopeptidase, CALLA, CD10, Enkephalinase, EPN, membrane metallo endopeptidase, MME, MME,CD10, NEP, Neprilysin, Neutral endopeptidase, Neutral endopeptidase 24.11

Validation Data Gallery

Filter:
  • Immunohistochemistry (IHC) staining of human kidney tissue using MME,CD10 Polyclonal antibody (23782-1-AP)

    IHC staining of human kidney using 23782-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 23782-1-AP (CD10,MME Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using MME,CD10 Polyclonal antibody (23782-1-AP)

    IHC staining of human kidney using 23782-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 23782-1-AP (CD10,MME Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human renal cell carcinoma tissue using MME,CD10 Polyclonal antibody (23782-1-AP)

    IHC staining of human renal cell carcinoma using 23782-1-AP

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 23782-1-AP (CD10,MME Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human renal cell carcinoma tissue using MME,CD10 Polyclonal antibody (23782-1-AP)

    IHC staining of human renal cell carcinoma using 23782-1-AP

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 23782-1-AP (CD10,MME Antibody) at dilution of 1:200 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of human kidney tissue using MME,CD10 Polyclonal antibody (23782-1-AP)

    IF Staining of human kidney using 23782-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using MME,CD10 antibody (23782-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of human kidney tissue using MME,CD10 Polyclonal antibody (23782-1-AP)

    IF Staining of human kidney using 23782-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using MME,CD10 antibody (23782-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Flow cytometry (FC) experiment of Jurkat cells using MME,CD10 Polyclonal antibody (23782-1-AP)

    FC experiment of Jurkat using 23782-1-AP

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human MME,CD10 (23782-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IHC detected inhuman kidney tissue, human renal cell carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman kidney tissue
Positive FC detected inJurkat cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

23782-1-AP targets MME,CD10 in IHC, IF, FC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MME,CD10 fusion protein Ag20634
Full Name membrane metallo-endopeptidase
Calculated Molecular Weight 750 aa, 86 kDa
GenBank Accession NumberBC101632
Gene Symbol MME
Gene ID (NCBI) 4311
RRIDAB_2879323
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MME is also names as neprilysin,CALLA,NEP,SFE,CD10 and belongs to the peptidase M13 family.It is a glycoprotein that is particularly abundant in kidney, where it is present on the brush border of proximal tubules and on glomerular epithelium.CD10 has been considered a useful marker in the diagnosis of renal carcinomas, because of its expression in clear cell and papillary renal cell carcinomas and its absence in chromophobe renal cell carcinomas(PMID:15286660).

Protocols

Product Specific Protocols
IHC protocol for MME,CD10 antibody 23782-1-APDownload protocol
IF protocol for MME,CD10 antibody 23782-1-APDownload protocol
FC protocol for MME,CD10 antibody 23782-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols