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CD100 Monoclonal antibody

CD100 Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

CloneNo.

1C1A6

Cat no : 66582-1-Ig

Synonyms

A8, BB18, C9orf164, CD100, coll 4, GR3, M sema G, SEMA4D, SEMAJ, Semaphorin 4D



Tested Applications

Positive WB detected inHL-60 cells, HeLa cells, K-562 cells, THP-1 cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:3000
Immunohistochemistry (IHC)IHC : 1:200-1:500
Sample-dependent, check data in validation data gallery

Product Information

66582-1-Ig targets CD100 in WB, IHC,ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CD100 fusion protein Ag26461
Full Name sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D
Calculated molecular weight 96 kDa
Observed molecular weight 150 kDa
GenBank accession numberBC054500
Gene symbol SEMA4D
Gene ID (NCBI) 10507
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD100 (also known as SEMA4D), a 150 kDa homodimer, belongs to the family of immune semaphorins, and CD100 is abundantly expressed on resting T cells, NK cells, and antigen-presenting cells. As a transmembrane glycoprotein, it is digested to its soluble form, sCD100, by specific matrix metalloproteinases. CD100 has been reported that the expression levels of CD100, and one of its receptors, plexin-B1 (PLXNB1), are increased in head and neck, prostate, colon, breast, and lung cancers, and the interaction between them provides oncogenic signaling essential for tumor growth and metastasis.

Protocols

Product Specific Protocols
WB protocol for CD100 antibody 66582-1-IgDownload protocol
IHC protocol for CD100 antibody 66582-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols