LAMP1 Antibody

LAMP1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 21997-1-AP

Synonyms

CD107a, LAMP 1, LAMP1, LAMPA, LGP120



Tested Applications

Positive WB detected inU-937 cells
Positive IHC detected inhuman gliomas tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The 21997-1-AP immunogen is Fusion Protein LAMP1 protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity human, turtles
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LAMP1 fusion protein Ag17367
Full Name lysosomal-associated membrane protein 1
Calculated molecular weight 417aa,45 kDa
Observed molecular weight 120 kDa
GenBank accession number BC021288
Gene symbol LAMP1
Gene ID (NCBI) 3916
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

LAMP1 (also known as CD107a) is a 90-120 kDa heavily glycosylated membrane protein enriched in the lysosomal membrane. LAMP1 functions to provide selectins with carbohydrate ligands. This protein has also been shown to be a marker of degranulation on lymphocytes such as CD8+ and NK cells and may also play a role in tumor cell differentiation and metastasis.

Protocols

Product Specific Protocols
WB protocol for LAMP1 antibody 21997-1-APDownload protocol
IHC protocol for LAMP1 antibody 21997-1-APDownload protocol
IF protocol for LAMP1 antibody 21997-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Fish Shellfish Immunol

Identification and functional characterization of interferon-γ-inducible lysosomal thiol reductase (GILT) gene in common Chinese cuttlefish Sepiella japonica.

Authors - Zan Pang
humanFC

Cancer Lett

Novel half-sandwich iridium OˆC (carbene)-Complexes: In vitro and in vivo tumor growth suppression and pro-apoptosis via ROS-mediated cross-talk between mitochondria and lysosomes.

Authors - Zhishan Xu
humanWB

Cell Prolif

PMID: 31033054

Authors - Jiyao Sheng
mouseIHC

Neurobiol Dis

Dysregulated autophagy as a new aspect of the molecular pathogenesis of Krabbe disease.

Authors - Ambra Del Grosso
humanWB

Oxid Med Cell Longev

Enhanced p62-NRF2 Feedback Loop due to Impaired Autophagic Flux Contributes to Arsenic-Induced Malignant Transformation of Human Keratinocytes.

Authors - Xiafang Wu
humanIHC

Pathol Res Pract

Altered expression of lysosomal associated membrane protein 1 in esophageal squamous cell carcinoma.

Authors - Jian Huang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Mayru (Verified Customer) (04-30-2019)

The antibody works great for detection of LAMP1 protein expression using Western blot analysis.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: H4 cells
No image submitted with review