|Positive WB detected in||U-937 cells|
|Positive IHC detected in||human gliomas tissue, human pancreas tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
The 21997-1-AP immunogen is Fusion Protein LAMP1 protein expressed in E. coli.
|Cited Reactivity||human, turtles|
|Host / Isotype||Rabbit / IgG|
|Immunogen||LAMP1 fusion protein Ag17367|
|Full Name||lysosomal-associated membrane protein 1|
|Calculated molecular weight||417aa,45 kDa|
|Observed molecular weight||120 kDa|
|GenBank accession number||BC021288|
|Gene ID (NCBI)||3916|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
LAMP1 (also known as CD107a) is a 90-120 kDa heavily glycosylated membrane protein enriched in the lysosomal membrane. LAMP1 functions to provide selectins with carbohydrate ligands. This protein has also been shown to be a marker of degranulation on lymphocytes such as CD8+ and NK cells and may also play a role in tumor cell differentiation and metastasis.
Fish Shellfish Immunol
Identification and functional characterization of interferon-γ-inducible lysosomal thiol reductase (GILT) gene in common Chinese cuttlefish Sepiella japonica.
Novel half-sandwich iridium OˆC (carbene)-Complexes: In vitro and in vivo tumor growth suppression and pro-apoptosis via ROS-mediated cross-talk between mitochondria and lysosomes.
Dysregulated autophagy as a new aspect of the molecular pathogenesis of Krabbe disease.
Oxid Med Cell Longev
Enhanced p62-NRF2 Feedback Loop due to Impaired Autophagic Flux Contributes to Arsenic-Induced Malignant Transformation of Human Keratinocytes.
Pathol Res Pract
Altered expression of lysosomal associated membrane protein 1 in esophageal squamous cell carcinoma.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Mayru (Verified Customer) (04-30-2019)
The antibody works great for detection of LAMP1 protein expression using Western blot analysis.