Tested Applications
| Positive WB detected in | Mo7e cells |
| Positive IHC detected in | human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Mo7e cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
84259-5-RR targets CD117/c-Kit in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Recombinant Protein Predict reactive species |
| Full Name | v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog |
| Calculated Molecular Weight | 976 aa, 110 kDa |
| Observed Molecular Weight | 140 kDa |
| GenBank Accession Number | BC071593 |
| Gene Symbol | CD117 |
| Gene ID (NCBI) | 3815 |
| RRID | AB_3671807 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purfication |
| UNIPROT ID | P10721 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CD117, also known as c-Kit and SCFR, is a transmembrane protein with tyrosine kinase activity encoded by the oncogene c-kit (PMID: 2448137). It is a member of the type III receptor tyrosine kinase family, which also includes CSF-1R, PDGFRβ, PDGFRα, and FLT3 (PMID: 29518044). CD117 is expressed on hematopoietic stem cells and progenitor cells, mast cells, and is also found in a wide range of non-haemopoietic cell types (including melanocytes, germ cells, astrocytes, renal tubules, breast glandular epithelial cells, sweat glands, and interstitial cells of Cajal) (PMID: 10582338; 23073628). CD117 plays an important role in early hemopoiesis. It is also involved in pigmentation, fertility, gut movement, and some aspects of the nervous system (PMID: 23073628).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CD117/c-Kit antibody 84259-5-RR | Download protocol |
| IHC protocol for CD117/c-Kit antibody 84259-5-RR | Download protocol |
| WB protocol for CD117/c-Kit antibody 84259-5-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CD117/c-Kit Recombinant monoclonal antibody (241313C5), catalog number 84259-5-RR, has 3 total citations. These appear in Cell Death Discovery, Cells, and iScience. The associated research fields include oocyte actin cortex biology, PI3K signaling and imatinib resistance in head and neck mucosal melanoma, and mast cell parthanatos in allergic rhinitis.
| Species | Application | Title |
|---|---|---|
Cell Death Discov Zona pellucida is required for oocyte actin cortex and oocyte-somatic cell interactions during oocyte growth. | ||
Cells Stemness and Survival: CD117(+)/CD133(+) Subpopulations Sustain PI3K Signaling and Drive Imatinib Resistance in Head and Neck Mucosal Melanoma. | ||
iScience PBX3 regulates mast cell parthanatos via TOP2A mediated DNA damage in allergic rhinitis. |
