CD14 Polyclonal antibody

CD14 Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 17000-1-AP

Synonyms

CD14, CD14 molecule



Tested Applications

Positive WB detected inHL-60 cells, RAW 264.7 cells, human spleen tissue
Positive IHC detected inhuman hepatocirrhosis tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inRAW 264.7 cells
Positive FC detected inRAW 264.7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

17000-1-AP targets CD14 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, mouse, RAT, swine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD14 fusion protein Ag10693
Full Name CD14 molecule
Calculated molecular weight 375 aa, 40 kDa
Observed molecular weight 50-55 kDa
GenBank accession numberBC010507
Gene symbol CD14
Gene ID (NCBI) 929
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD14 is a 50-55 kDa glycosylphosphatidylinositol-anchored glycoprotein. CD14 is preferentially expressed on monocytes and macrophages. CD14 acts as a co-receptor (along with TLR4 and MD-2) for bacterial liposaccharides (LPS). It plays a major role in the inflammatory response of monocytes to LPS.

Protocols

Product Specific Protocols
WB protocol for CD14 antibody 17000-1-APDownload protocol
IHC protocol for CD14 antibody 17000-1-APDownload protocol
IF protocol for CD14 antibody 17000-1-APDownload protocol
FC protocol for CD14 antibody 17000-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Biomed Pharmacother

Short-interval exposure to ambient fine particulate matter (PM2.5) exacerbates the susceptibility of pulmonary damage in setting of lung ischemia-reperfusion injury in rodent: Pharmacomodulation of melatonin.

Authors - Fan-Yen Lee
ratIHC,IF

Am J Transl Res

Adipose-derived mesenchymal stem cell-derived exosomes markedly protected the brain against sepsis syndrome induced injury in rat.

Authors - Chia-Lo Chang
humanIF

Br J Sports Med

Chronic inflammation is a feature of Achilles tendinopathy and rupture.

Authors - Stephanie Georgina Dakin
humanWB

Virchows Arch

Immune cell landscape in therapy-naïve squamous cell and adenocarcinomas of the lung.

Authors - Luka Brcic
humanWB

Acta Physiol (Oxf)

Acute physical exercise and long-term individual shear rate therapy increase telomerase activity in human peripheral blood mononuclear cells.

Authors - A Zietzer
ratIF

J Neuropathol Exp Neurol

Combined Adipose-Derived Mesenchymal Stem Cells and Low-Energy Extracorporeal Shock Wave Therapy Protect the Brain From Brain Death-Induced Injury in Rat.

Authors - Kuan-Hung Chen