Filter: AllWBIHCIF-PIF/ICC

WB analysis using 60253-1-Ig
Untreated and PNGase F-treated lysates of human milk were subjected to SDS PAGE followed by western blot with 60253-1-Ig (CD14 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of THP-1 using 60253-1-Ig
THP-1 cells were subjected to SDS PAGE followed by western blot with 60253-1-Ig (CD14 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of human placenta using 60253-1-Ig
human placenta tissue were subjected to SDS PAGE followed by western blot with 60253-1-Ig (CD14 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of RAW 264.7 using 60253-1-Ig
RAW264.7 cells were subjected to SDS PAGE followed by western blot with 60253-1-Ig (CD14 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of human tonsillitis using 60253-1-Ig
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60253-1-Ig (CD14 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human tonsillitis using 60253-1-Ig
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60253-1-Ig (CD14 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of human tonsillitis using 60253-1-Ig
Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD14 antibody (60253-1-Ig, Clone: 2C1D9 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
IF Staining of RAW 264.7 using 60253-1-Ig
Immunofluorescent analysis of RAW 264.7 cells using 60253-1-Ig (CD14 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).

CD14 Monoclonal antibody

CD14 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Cat No. 60253-1-Ig

Clone No.2C1D9

Publications(18)

Reviews (3)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Synonyms

Myeloid cell-specific leucine-rich glycoprotein, Monocyte differentiation antigen CD14, urinary form, Monocyte differentiation antigen CD14, membrane-bound form, CD14 molecule, 2C1D9

Formulation: PBS and Azide

PBS and Azide

PBS Only

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Publications (18)
Protocols
Reviews (3)

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	THP-1 cells, human milk, human placenta tissue, RAW 264.7 cells
Positive IHC detected in	human tonsillitis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	human tonsillitis tissue
Positive IF/ICC detected in	RAW 264.7 cells, human tonsillitis tissue

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:1000-1:4000
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 5 publications below
IHC	See 6 publications below
IF	See 6 publications below
FC	See 1 publications below

Product Information

60253-1-Ig targets CD14 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse, rabbit
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	CD14 fusion protein Ag10693 Predict reactive species
Full Name	CD14 molecule
Calculated Molecular Weight	375 aa, 40 kDa
Observed Molecular Weight	45-55 kDa
GenBank Accession Number	BC010507
Gene Symbol	CD14
Gene ID (NCBI)	929
ENSEMBL Gene ID	ENSG00000170458
RRID	AB_2881374
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein G purification
UNIPROT ID	P08571
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD14 is a 50-55 kDa glycosylphosphatidylinositol-anchored glycoprotein preferentially expressed on monocytes and macrophages, and at lower levels on granulocytes (PMID: 3385210; 2462937; 7685797). CD14 can also exist as a soluble protein. CD14 acts as a co-receptor for bacterial liposaccharides (LPS) (PMID: 1698311). It plays a major role in the inflammatory response of monocytes to LPS.

Protocols

Product Specific Protocols
WB protocol for CD14 antibody 60253-1-Ig	Download protocol
IHC protocol for CD14 antibody 60253-1-Ig	Download protocol
IF protocol for CD14 antibody 60253-1-Ig	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IHC	Mol Cell Lactylation-driven METTL3-mediated RNA m6A modification promotes immunosuppression of tumor-infiltrating myeloid cells. Authors - Jia Xiong View Article
human	IF	Arthritis Rheumatol Attenuation of rheumatoid arthritis through the inhibition of caspase3/GSDME-mediated pyroptosis induced by TNF-α. Authors - Zeqing Zhai View Article
rabbit	IF	Bioact Mater Novel magnetic silk fibroin scaffolds with delayed degradation for potential long-distance vascular repair. Authors - Xin Liu View Article
mouse	WB,IF	Sci Rep High-Throughput Sequencing and Co-Expression Network Analysis of lncRNAs and mRNAs in Early Brain Injury Following Experimental Subarachnoid Haemorrhage. Authors - Jianhua Peng View Article
mouse		Front Pharmacol Achyranthes bidentata Polysaccharide Activates Nuclear Factor-Kappa B and Promotes Cytokine Production in J774A.1 Cells Through TLR4/MyD88 Signaling Pathway Authors - Sairong Fan View Article
human	IHC	J Cancer MMP9 and IGFBP1 Regulate Tumor Immune and Drive Tumor Progression in Clear Cell Renal Cell Carcinoma. Authors - Tianbo Xu View Article

Reviews

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The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH Sareena (Verified Customer) (06-06-2023)	Works well when conjugated to fluorophores and tested with compensation beads Applications: Immunofluorescence, Flow Cytometry Primary Antibody Dilution: CD14 Monoclonal antibody
FH Sunil (Verified Customer) (05-15-2023)	Great and fast delivery which is always useful! Applications: Flow Cytometry
FH Emma (Verified Customer) (11-29-2021)	Works well by IF on FFPE tissue. Tris-EDTA antigen retrieval pH 9.0. Applications: Immunofluorescence Primary Antibody Dilution: 1:50 Cell Tissue Type: Prostate CD14 Antibody Immunofluorescence validation (1:50 dilution) in Prostate (Cat no:60253-1-Ig)