CD14 Monoclonal antibody

CD14 Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, FC, Cell treatment, ELISA

Conjugate

Unconjugated

CloneNo.

2C1D9

Cat no : 60253-1-Ig

Synonyms

CD14, CD14 molecule



Tested Applications

Positive WB detected inTHP-1 cells, RAW 264.7 cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inRAW 264.7 cells, human tonsillitis tissue
Positive FC detected inTHP-1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 60253-1-Ig is CD14 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CD14 fusion protein Ag10693
Full Name CD14 molecule
Calculated Molecular Weight 375 aa, 40 kDa
Observed Molecular Weight 55 kDa
GenBank Accession NumberBC010507
Gene Symbol CD14
Gene ID (NCBI) 929
RRIDAB_2881374
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD14 is a 50-55 kDa glycosylphosphatidylinositol-anchored glycoprotein preferentially expressed on monocytes and macrophages, and at lower levels on granulocytes (PMID: 3385210; 2462937; 7685797). CD14 can also exist as a soluble protein. CD14 acts as a co-receptor for bacterial liposaccharides (LPS) (PMID: 1698311). It plays a major role in the inflammatory response of monocytes to LPS.

Protocols

Product Specific Protocols
WB protocol for CD14 antibody 60253-1-IgDownload protocol
IHC protocol for CD14 antibody 60253-1-IgDownload protocol
IF protocol for CD14 antibody 60253-1-IgDownload protocol
FC protocol for CD14 antibody 60253-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Mol Cell

Lactylation-driven METTL3-mediated RNA m6A modification promotes immunosuppression of tumor-infiltrating myeloid cells.

Authors - Jia Xiong
humanIF

Arthritis Rheumatol

Attenuation of rheumatoid arthritis through the inhibition of caspase3/GSDME-mediated pyroptosis induced by TNF-α.

Authors - Zeqing Zhai
rabbitIF

Bioact Mater

Novel magnetic silk fibroin scaffolds with delayed degradation for potential long-distance vascular repair.

Authors - Xin Liu
mouseWB,IF

Sci Rep

High-Throughput Sequencing and Co-Expression Network Analysis of lncRNAs and mRNAs in Early Brain Injury Following Experimental Subarachnoid Haemorrhage.

Authors - Jianhua Peng
mouseCell treatment

Front Pharmacol

Achyranthes bidentata Polysaccharide Activates Nuclear Factor-Kappa B and Promotes Cytokine Production in J774A.1 Cells Through TLR4/MyD88 Signaling Pathway

Authors - Sairong Fan
humanIHC

J Cancer

MMP9 and IGFBP1 Regulate Tumor Immune and Drive Tumor Progression in Clear Cell Renal Cell Carcinoma.

Authors - Tianbo Xu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Sareena (Verified Customer) (06-06-2023)

Works well when conjugated to fluorophores and tested with compensation beads

  • Applications: Immunofluorescence, Flow Cytometry
  • Primary Antibody Dilution: CD14 Monoclonal antibody
FH

Sunil (Verified Customer) (05-15-2023)

Great and fast delivery which is always useful!

  • Applications: Flow Cytometry
FH

Emma (Verified Customer) (11-29-2021)

Works well by IF on FFPE tissue. Tris-EDTA antigen retrieval pH 9.0.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:50
  • Cell Tissue Type: Prostate
CD14 Antibody Immunofluorescence validation (1:50 dilution) in Prostate (Cat no:60253-1-Ig)