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CD14 Antibody 2 Publications

Mouse Monoclonal| Catalog number: 60253-1-Ig |CloneNo.: 2C1D9

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Con: 75 μg/150 μl

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Tested Applications

Applications:
WB, IHC, IF, ELISA
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Images See all

CD14 Antibody WB THP-1 cells 60253-1-Ig

THP-1 cells were subjected to SDS PAGE followed by western blot with 60253-1-Ig (CD14 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

CD14 Antibody IHC human tonsillitis tissue 60253-1-Ig

Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60253-1-Ig( CD14 Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

CD14 Antibody IHC human tonsillitis tissue 60253-1-Ig

Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60253-1-Ig( CD14 Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

CD14 Antibody IF RAW 264.7 cells 60253-1-Ig

Immunofluorescent analysis of RAW 264.7 cells using 60253-1-Ig(CD14 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L)

Species specificity:
human, mouse
Positive WB detected in:
THP-1 cells, RAW 264.7 cells
Positive IHC detected in:
human tonsillitis tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
RAW 264.7 cells
Recommended dilution:
WB : 1:500-1:2000
IHC : 1:50-1:500
IF : 1:50-1:500
Sample-dependent, check data in Validation tab

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Mouse
Purification method:
Protein G purification
Isotype:
IgG1
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
CD14 fusion protein Ag10693
Full name:
CD14 molecule
Calculated molecular weight:
375aa,40 kDa
Observed molecular weight:
55 kDa
GenBank accession number:
BC010507
Gene ID (NCBI):
929
Gene symbol
CD14
Synonyms
CD14, CD14 molecule

Background

CD14 is a 50-55 kDa glycosylphosphatidylinositol-anchored glycoprotein. CD14 is preferentially expressed on monocytes and macrophages. CD14 acts as a co-receptor (along with TLR4 and MD-2) for bacterial liposaccharides (LPS). It plays a major role in the inflammatory response of monocytes to LPS.


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