CD14 Monoclonal antibody
CD14 Monoclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
CloneNo.
2C1D9
Cat no : 60253-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | THP-1 cells, RAW 264.7 cells |
| Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | RAW 264.7 cells, human tonsillitis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 1 publications below |
| IF | See 1 publications below |
Product Information
The immunogen of 60253-1-Ig is CD14 Fusion Protein expressed in E. coli.
| Tested Reactivity | human, mouse |
| Cited Reactivity | mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | CD14 fusion protein Ag10693 |
| Full Name | CD14 molecule |
| Calculated molecular weight | 375 aa, 40 kDa |
| Observed molecular weight | 55 kDa |
| GenBank accession number | BC010507 |
| Gene symbol | CD14 |
| Gene ID (NCBI) | 929 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CD14 is a 50-55 kDa glycosylphosphatidylinositol-anchored glycoprotein. CD14 is preferentially expressed on monocytes and macrophages. CD14 acts as a co-receptor (along with TLR4 and MD-2) for bacterial liposaccharides (LPS). It plays a major role in the inflammatory response of monocytes to LPS.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CD14 antibody 60253-1-Ig | Download protocol |
| IHC protocol for CD14 antibody 60253-1-Ig | Download protocol |
| IF protocol for CD14 antibody 60253-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Med Rep Sphingomyelin synthase 2 affects CD14‑associated induction of NF‑κB by lipopolysaccharides in acute lung injury in mice. | ||
Sci Rep High-Throughput Sequencing and Co-Expression Network Analysis of lncRNAs and mRNAs in Early Brain Injury Following Experimental Subarachnoid Haemorrhage. |
