Biotin-conjugated CD40L/CD154 Monoclonal antibody

CD40L/CD154 Monoclonal Antibody for IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

IHC, ELISA

Conjugate

Biotin Fluorescent Dye

CloneNo.

1E6D10

Cat no : Biotin-66502

Synonyms

CD154, CD40 L, CD40 ligand, CD40L, CD40LG, gp39, hCD40L, HIGM1, IGM, IMD3, T BAM, T cell antigen Gp39, TNF related activation protein, TNFSF5, TRAP



Tested Applications

Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

Biotin-66502 targets CD40L/CD154 in IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen CD40L/CD154 fusion protein Ag24941
Full Name CD40 ligand
Calculated molecular weight 261 aa, 29 kDa
GenBank accession numberBC071754
Gene symbol CD40LG
Gene ID (NCBI) 959
Conjugate Biotin Fluorescent Dye
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The CD40 ligand (CD40L, TRAP, CD154), a member of the TNF superfamily of ligands, is expressed as either a 33-kd transmembrane homologue or 18-kd soluble form (sCD154). CD40L is primarily expressed on activated CD4+ T cells and on a small proportion of CD8+ T cells and platelets. It binds to CD40 on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. Recent studies have suggested that CD40/CD40L interactions regulate oxidative stress and affect various signaling pathways in both the immunological and the cardiovascular systems. The CD40/CD40L system is also involved in tumorigenesis. Its expression is tightly regulated, and abnormal levels of CD40L are associated with the pathogenesis of atheromatous plaque destabilization and thrombotic events. Multiple mutations in CD40LG gene have been identified that are associated with hyper-IgM immunodeficiency syndrome type 1.

Protocols

Product Specific Protocols
IHC protocol for Biotin-conjugated CD40L/CD154 antibody Biotin-66502Download protocol
Standard Protocols
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