Tested Applications
| Positive WB detected in | CTLL-2 cells, EL-4 cells, mouse spleen tissue |
| Positive IF/ICC detected in | EL-4-B5 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
83871-1-RR targets CD48 in WB, IF/ICC, ELISA applications and shows reactivity with mouse samples.
| Tested Reactivity | mouse |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Recombinant protein Predict reactive species |
| Full Name | CD48 antigen |
| Calculated Molecular Weight | 27 kDa |
| Observed Molecular Weight | 45-60 kDa |
| GenBank Accession Number | BAE96326.1 |
| Gene Symbol | Cd48 |
| Gene ID (NCBI) | 12506 |
| RRID | AB_3671457 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purfication |
| UNIPROT ID | P18181 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Signaling lymphocytic activation molecule family 2 (SLAMF2, CD48) is an adhesion and costimulatory molecule expressed constitutively on most hematopoietic cells, particularly in antigen-presenting cells (APC). CD48 is expressed on all the hematopoietic cells, both in humans and mice, except for murine neutrophils and long-term HSC. CD48 exists as both a membrane-bound (mCD48) and a soluble (sCD48) form. CD48 can activate T cells, antigen-presenting cells and granulocytes, by binding to CD2 or bacterial FimH, and through cell intrinsic effects. Interactions between CD48 and its high-affinity ligand CD244 are more complex, with both stimulatory and inhibitory outcomes. CD48 expression is increased in autoimmunity and allergy diseases, and anti-CD48 monoclonal antibodies have been shown to attenuate experimental autoimmune encephalomyelitis.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CD48 antibody 83871-1-RR | Download protocol |
| WB protocol for CD48 antibody 83871-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CD48 Recombinant monoclonal antibody (240732E3), catalog number 83871-1-RR, has a total of 3 citations. These appear in International Journal of Biological Macromolecules, Frontiers in Immunology, and Materials Today Bio. The associated research fields span hepatocellular carcinoma treatment, reproductive immunology (recurrent pregnancy loss), and oncology pharmacology (CDK4/6 inhibitor-induced myelosuppression). All citations used mouse species with WB or IF applications.
| Species | Application | Title |
|---|---|---|
Int J Biol Macromol Structural characterisation of a polysaccharide from Sanghuangporus vaninii and its synergistic anti-hepatocellular carcinoma activity in combination with sorafenib. | ||
Front Immunol Dysfunctional decidual CD2(+)CD4(+)T cells regulated by Rev-erbα - GFPT1 - GPI anchored CD58 axis of decidual stromal cells underlies sleep disturbance induced recurrent pregnancy loss. | ||
Mater Today Bio Porosity-engineered long-acting protopanaxadiol microspheres prevent CDK4/6 inhibitor-induced myelosuppression. |
