Tested Applications
| Positive WB detected in | RAW 264.7 cells, J774A.1 cells, NR8383 cells |
| Positive IHC detected in | mouse spleen tissue, mouse liver tissue, rat small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse spleen tissue, rat small intestine tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
83014-5-RR targets CD68 in WB, IHC, IF-P, ELISA applications and shows reactivity with mouse, rat samples.
| Tested Reactivity | mouse, rat |
| Cited Reactivity | mouse, rat, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Eg0787 Product name: Recombinant mouse CD68 protein Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS Tag: C-6*HIS Domain: 21-291 aa of NM_001291058.1 Sequence: DCPHKKAVTLLPSFTMTPTATESTASPTTSHRPTTTSHGNVTVHTSSGPTTVTHNPATTTSHGNATISHATVSPTTNGTATSPRSSTVGPHPGPPPPSPSPRSKGALGNYTWANGSQPCVQLQAQIQIRILYPIQGGRKAWGISVLNPNKTKVQGGCDGTHPHLSLSFPYGQLTFGFKQDLHQSPSTVYLDYMAVEYNVSFPQAAQWTFMAQNSSLRELQAPLGQSFCCGNASIVLSPAVHLDLLSLRLQAAQLPDKGHFGPCFSCNRDQS Predict reactive species |
| Full Name | CD68 antigen |
| Calculated Molecular Weight | 35 kDa |
| Observed Molecular Weight | 100-120 kDa |
| GenBank Accession Number | NM_001291058.1 |
| Gene Symbol | Cd68 |
| Gene ID (NCBI) | 12514 |
| RRID | AB_3670757 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purfication |
| UNIPROT ID | P31996-1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Mouse CD68 (also known as macrosialin) is a type I transmembrane glycoprotein that is highly and specifically expressed by mouse tissue macrophages, and to a lesser extent by dendritic cells. It belongs to the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family and primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. CD68 is also a member of the scavenger receptor family. It may play a role in phagocytic activities of tissue macrophages. Due to heavy glycosylation, the apparent molecular mass of CD68 detected by Western blot is generally observed between 80 and 140 kDa, far exceeding its unmodified core protein weight of ~34-37 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CD68 antibody 83014-5-RR | Download protocol |
| IHC protocol for CD68 antibody 83014-5-RR | Download protocol |
| WB protocol for CD68 antibody 83014-5-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
CD68 Recombinant monoclonal antibody (230504G10), catalog no. 83014-5-RR, has 16 citations across journals: J Adv Res, Mater Today Bio, Acta Biomater, Clin Transl Med, ACS Appl Mater Interfaces, J Agric Food Chem, Transl Res, Commun Biol, Int Immunopharmacol, Front Pharmacol, J Nutr Biochem, Mol Pharm, FASEB J, J Nat Prod, and Lupus. Research fields include wound healing, neuroinflammation, cardiac injury, atherosclerosis, hepatocellular carcinoma, tissue engineering, and immunology.
| Species | Application | Title |
|---|---|---|
Mater Today Bio Customized ultra-flexible and adhesive spinal cord stimulation electrode for chronic pain management. | ||
Mater Today Bio Engineered human plasma-derived cryogels as a multifunctional scaffold to promote diabetic wound healing. | ||
Acta Biomater Adhesive polyethylene glycol hydrogels with metformin enabling in-situ drug delivery reprogramming immuno-metabolism for tissue repair in diabetic foot ulcers. | ||
Clin Transl Med TALDO1 promotes lipid metabolic reprogramming and immunosuppressive microenvironment remodelling in hepatocellular carcinoma | ||
ACS Appl Mater Interfaces A Fibrous-Porous Microsphere-Based Composite Filler for Synchronized Immediate and Long-Term Soft Tissue Restoration. |
Reviews
What customers say
One reviewer (Zakia Z.) gave this CD68 recombinant rabbit monoclonal antibody a 5-star rating after testing it on mouse brain tissue by Western Blot at 1:2000 dilution. She reported strong signal intensity and high specificity, noting that while a broad smear was not clearly visible due to her own membrane transfer issues, she was confident that protocol optimization would yield clean results. She highlighted excellent reactivity with mouse tissue and recommended it for neural and macrophage tracking studies.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Zakia (Verified Customer) (08-04-2026) | We tested this CD68 recombinant rabbit monoclonal antibody (Cat No. 83014-5-RR) on mouse brain samples using Western Blot. Overall, the performance was very good. Even though the expected broad smear/band is not clearly visible in our current blot due to our own membrane transfer issues, the signal intensity and specificity look highly promising. We are confident that resolving our optimization and transfer protocol will yield excellent, clean results. It shows great reactivity with mouse tissue, and we highly recommend it for neural and macrophage tracking studies.
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