Tested Applications
| Positive IF-P detected in | mouse spleen tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-83014-5 targets CD68 in IF-P applications and shows reactivity with mouse, rat samples.
| Tested Reactivity | mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Eg0787 Product name: Recombinant mouse CD68 protein Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS Tag: C-6*HIS Domain: 21-291 aa of NM_001291058.1 Sequence: DCPHKKAVTLLPSFTMTPTATESTASPTTSHRPTTTSHGNVTVHTSSGPTTVTHNPATTTSHGNATISHATVSPTTNGTATSPRSSTVGPHPGPPPPSPSPRSKGALGNYTWANGSQPCVQLQAQIQIRILYPIQGGRKAWGISVLNPNKTKVQGGCDGTHPHLSLSFPYGQLTFGFKQDLHQSPSTVYLDYMAVEYNVSFPQAAQWTFMAQNSSLRELQAPLGQSFCCGNASIVLSPAVHLDLLSLRLQAAQLPDKGHFGPCFSCNRDQS Predict reactive species |
| Full Name | CD68 antigen |
| Calculated Molecular Weight | 35 kDa |
| Observed Molecular Weight | 100-120 kDa |
| GenBank Accession Number | NM_001291058.1 |
| Gene Symbol | Cd68 |
| Gene ID (NCBI) | 12514 |
| RRID | AB_3747354 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Excitation Laser | Blue laser (488 nm) |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P31996-1 |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Mouse CD68 (also known as macrosialin) is a type I transmembrane glycoprotein that is highly and specifically expressed by mouse tissue macrophages, and to a lesser extent by dendritic cells. It belongs to the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family and primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. CD68 is also a member of the scavenger receptor family. It may play a role in phagocytic activities of tissue macrophages.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 CD68 antibody CL488-83014-5 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Reviews
What customers say
One customer rated this CoraLite 488-conjugated antibody 4 out of 5 stars for immunofluorescence on adult mouse liver FFPE tissue. The antibody was diluted 1:250 and incubated overnight after blocking. The sample was co-stained with Lyve1 (magenta) and DAPI (blue), and the reviewer reported satisfactory staining results in their multiplex IF protocol.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
bhavin (Verified Customer) (12-16-2025) | the coralite ab (AF488) was incubated ON after blocking (adult mouse liver tissue). The sample was co-stained with lyve1 (magenta) and Dapi (blue)
 |
