CD86 (C-terminal) Polyclonal antibody

CD86 (C-terminal) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 26903-1-AP

Synonyms

Activation B7 2 antigen, B7 2, B70, CD28LG2, CD86, CD86 (C-terminal), CD86 molecule, CTLA 4 counter receptor B7.2, FUN 1, LAB72



Tested Applications

Positive WB detected inDaudi cells, Raji cell, Ramos cells, RAW 264.7 cells, LPS-treated RAW 264.7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

26903-1-AP targets CD86 (C-terminal) in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD86 (C-terminal) fusion protein Ag25432
Full Name CD86 molecule
Calculated molecular weight 329 aa, 38 kDa
Observed molecular weight 65 kDa
GenBank accession numberBC040261
Gene symbol CD86
Gene ID (NCBI) 942
RRIDAB_2880677
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD86 (also known as B7.2) is a costimulatory molecule belonging to the immunoglobulin superfamily. Primarily expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, and macrophages, CD86 is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response.

Protocols

Product Specific Protocols
WB protocol for CD86 (C-terminal) antibody 26903-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nat Commun

A photo-triggered self-accelerated nanoplatform for multifunctional image-guided combination cancer immunotherapy

Authors - Xiaoying Kang
ratWB,IF

Int J Biol Macromol

A multifunctional polydopamine/genipin/alendronate nanoparticle licences fibrin hydrogels osteoinductive and immunomodulatory potencies for repairing bone defects

Authors - Wentao Shi
humanWB

Cancer Immunol Immunother

Novel roles of VAT1 expression in the immunosuppressive action of diffuse gliomas.

Authors - Pei Yang
mouseIF

Front Bioeng Biotechnol

Heparin/Collagen-REDV Modification of Expanded Polytetrafluoroethylene Improves Regional Anti-thrombosis and Reduces Foreign Body Reactions in Local Tissues

Authors - Yaping Shan
mouseWB

Biochem Biophys Res Commun

Esomeprazole inhibits endoplasmic reticulum stress and ameliorates myocardial ischemia-reperfusion injury

Authors - Guoxiang Zhou
human,mouseWB,IF

Cell Death Differ

TRAF3/STAT6 axis regulates macrophage polarization and tumor progression

Authors - Jian-Hong Shi