CD8a Antibody

CD8a Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 17335-1-AP

Synonyms



Tested Applications

Positive IHC detected inhuman tonsillitis tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The 17335-1-AP immunogen is Fusion Protein CD8a protein expressed in E. coli.

Reactivity human
Cited Species human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD8a fusion protein Ag11370
Full Name CD8a molecule
Calculated molecular weight 235aa,26 kDa
Observed molecular weight
GenBank accession number BC025715
Gene symbol CD8A
Gene ID (NCBI) 925
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20oC. Aliquoting is unnecessary for -20oC storage.

Background Information

CD8a is the alpha chain of CD8, a transmembrane glycoprotein that is predominantly expressed on the surface of cytotoxic T cells, and can also be found on natural killer cells, cortical thymocytes, and dendritic cells. CD8 serves as a co-receptor for the T cell receptor (TCR). Both CD8 and TCR recognize antigens displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. CD8 plays a role in T cell development and activation of mature T cells.

Publications

SpeciesApplicationTitle
humanIHC

J Physiol Biochem

α2,6-Sialylation promotes immune escape in hepatocarcinoma cells by regulating T cell functions and CD147/MMP signaling.

Authors - Liping Wang
humanIHC

World J Gastroenterol

Clinical significance of programmed death 1/programmed death ligand 1 pathway in gastric neuroendocrine carcinomas.

Authors - Min-Wei Yang
humanIHC

Front Oncol

Changes in Tumor-Infiltrating Lymphocytes and Vascular Normalization in Breast Cancer Patients After Neoadjuvant Chemotherapy and Their Correlations With DFS.

Authors - Qiong Wang
humanIF

Oncol Lett

Survival analysis with regard to PD-L1 and CD155 expression in human small cell lung cancer and a comparison with associated receptors.

Authors - Yaolin Xu
humanIHC

Virol Sin

Detection of EBV and HHV6 in the Brain Tissue of Patients with Rasmussen's Encephalitis.

Authors - Dong Liu
humanIHC

Sci Rep

Inhibiting the CD8+ T cell infiltration in the tumor microenvironment after radiotherapy is an important mechanism of radioresistance.

Authors - Hai-Yan Chen