CD90 Monoclonal antibody
CD90 Monoclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
Human, pig, rat, rabbit, mouse and More (2)
Applications
WB, IHC, IF, FC, ELISA
Conjugate
Unconjugated
CloneNo.
2D7D11
Cat no : 66766-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | fetal human brain tissue, rabbit brain tissue, U2OS cells, human kidney tissue, pig brain tissue, pig spleen tissue, pig colon tissue |
| Positive IHC detected in | human tonsillitis tissue, human colon cancer tissue, human gliomas tissue, human lung cancer tissue, mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | human tonsillitis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 6 publications below |
| IHC | See 5 publications below |
| IF | See 12 publications below |
| FC | See 4 publications below |
Product Information
66766-1-Ig targets CD90 in WB, IHC, IF, FC, ELISA applications and shows reactivity with Human, pig, rat, rabbit, mouse samples.
| Tested Reactivity | Human, pig, rat, rabbit, mouse |
| Cited Reactivity | human, mouse, rat, rabbit, bovine |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | CD90 fusion protein Ag25603 |
| Full Name | Thy-1 cell surface antigen |
| Calculated Molecular Weight | 161 aa, 18 kDa |
| Observed Molecular Weight | 28 kDa |
| GenBank Accession Number | BC065559 |
| Gene Symbol | THY1 |
| Gene ID (NCBI) | 7070 |
| RRID | AB_2882112 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CD90, also known as THY1, is a 25-35 kD protein that is expressed on 1-4% of human fetal liver cells, cord blood cells, and bone marrow cells. CD90 is one of the essential surface molecules expressed on human MSC from bone marrow and other sources. Activation of Thy-1 has been reported to promote T cell activation. It also affects numerous nonimmunologic biological processes, including cellular adhesion, neurite outgrowth, tumor growth, migration, and cell death.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CD90 antibody 66766-1-Ig | Download protocol |
| IHC protocol for CD90 antibody 66766-1-Ig | Download protocol |
| IF protocol for CD90 antibody 66766-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Mol Cell Biol Cancer-associated adipocytes-derived G-CSF promotes breast cancer malignancy via Stat3 signaling. | ||
Int J Mol Sci Expression of Cancer Stem Cell Markers EpCAM and CD90 Is Correlated with Anti- and Pro-Oncogenic EphA2 Signaling in Hepatocellular Carcinoma. | ||
Int J Biol Sci Cancer-associated adipocytes promote the invasion and metastasis in breast cancer through LIF/CXCLs positive feedback loop. | ||
Cell Commun Signal SENP1 regulates the transformation of lung resident mesenchymal stem cells and is associated with idiopathic pulmonary fibrosis progression. | ||
Cell Signal miR-296-5p suppresses stem cell potency of hepatocellular carcinoma cells via regulating Brg1/Sall4 axis. | ||
Front Cardiovasc Med Epicardium-Derived Tbx18+ CDCs Transplantation Improve Heart Function in Infarcted Mice. |
