CoraLite®488-conjugated CES1 Monoclonal antibody

CES1 Monoclonal Antibody for IF, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF, ELISA

Conjugate

CoraLite®488 Fluorescent Dye

CloneNo.

1C11C1

Cat no : CL488-67079

Synonyms

ACAT, Brain carboxylesterase hBr1, CEH, CES1, CES2, Cocaine carboxylesterase, Egasyn, HMSE, HMSE1, Liver carboxylesterase 1, PCE 1, REH, Retinyl ester hydrolase, Serine esterase 1, SES1, TGH, Triacylglycerol hydrolase



Tested Applications

Positive IF detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 67079-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL488-67079 targets CES1 in IF, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CES1 fusion protein Ag10647
Full Name carboxylesterase 1 (monocyte/macrophage serine esterase 1)
Calculated molecular weight 566 aa, 62 kDa
Observed molecular weight 60-63 kDa
GenBank accession numberBC012418
Gene symbol CES1
Gene ID (NCBI) 1066
Conjugate CoraLite®488 Fluorescent Dye
Excitation/Emission maxima wavelengths488 nm/515 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

CES1(liver carboxylesterase 1) is also named as CES2, SES1 and belongs to the type-B carboxylesterase/lipase family. The deduced 567-amino acid protein contains a putative 18-amino acid signal peptide and a characteristic C-terminal endoplasmic reticulum retention signal (HXEL). It is the major hydrolytic enzyme responsible for the metabolism of numerous therapeutic agents as well as endogenous substrates.Westernblot analysis demonstrated that CES1 is expressed in human liver microsomes (HLM) but not in human intestinal microsomes (HIM)(PMID:19185566).

Protocols

Product Specific Protocols
IF protocol for CL488-conjugated CES1 antibody CL488-67079Download protocol
Standard Protocols
Click here to view our Standard Protocols