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  • KD/KO Validated

Chromogranin B Antibody

Chromogranin B Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 14968-1-AP

Synonyms

CgB, CHGB, Chromogranin B, SCG1, Secretogranin 1, Secretogranin I, SgI



Tested Applications

Positive WB detected inPC-12 cells, RAW 264.7 cells, mouse brain tissue
Positive IHC detected inhuman pancreas tissue, mouse pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inPC-12 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

14968-1-AP targets Chromogranin B in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Chromogranin B fusion protein Ag6833
Full Name chromogranin B (secretogranin 1)
Calculated molecular weight 78 kDa
Observed molecular weight 100 kDa
GenBank accession number BC000375
Gene symbol CHGB
Gene ID (NCBI) 1114
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for Chromogranin B antibody 14968-1-APDownload protocol
IHC protocol for Chromogranin B antibody 14968-1-APDownload protocol
IF protocol for Chromogranin B antibody 14968-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Biosci Biotechnol Biochem

Identification of proteins that bind to the neuroprotective agent neoechinulin A.

Authors - Shinji Kamisuki
  • KD Validated
humanWB

J Proteome Res

Label-Free LC-MS/MS Proteomic Analysis of Cerebrospinal Fluid Identifies Protein/Pathway Alterations and Candidate Biomarkers for Amyotrophic Lateral Sclerosis.

Authors - Mahlon Angus Collins

J Cell Sci

Chromogranin B regulates early stage insulin granule trafficking from the Golgi in pancreatic islet β-cells.

Authors - Shelby C Bearrows