Tested Applications
| Positive IHC detected in | human pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
21432-1-AP targets CHIT1 in IHC, IF, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag16039 Product name: Recombinant human CHIT1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 117-466 aa of BC103695 Sequence: QTFVNSAIRFLRKYSFDGLDLDWEYPGSQGSPAVDKERFTTLVQDLANAFQQEAQTSGKERLLLSAAVPAGQTYVDAGYEVDKIAQNLDFVNLMAYDFHGSWEKVTGHNSPLYKRQEESGAAASLNVDAAVQQWLQKGTPASKLILGMPTYGRSFTLASSSDTRVGAPATGSGTPGPFTKEGGMLAYYEVCSWKGATKQRIQDQKVPYIFRDNQWVGFDDVESFKTKVSYLKQKGLGGAMVWALDLDDFAGFSCNQGRYPLIQTLRQELSLPYLPSGTPELEVPKPGQPSEPEHGPSPGQDTFCQGKADGLYPNPRERSSFYSCAGGRLFQQSCPTGLVFSNSCKCCTWN Predict reactive species |
| Full Name | chitinase 1 (chitotriosidase) |
| Calculated Molecular Weight | 466 aa, 52 kDa |
| Observed Molecular Weight | 55 kDa |
| GenBank Accession Number | BC103695 |
| Gene Symbol | CHIT1 |
| Gene ID (NCBI) | 1118 |
| RRID | AB_2878857 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q13231 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for CHIT1 antibody 21432-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CHIT1 Polyclonal antibody (Cat# 21432-1-AP) has 3 citations published in Theranostics, Computational and Structural Biotechnology Journal, and Translational Andrology and Urology. The associated research fields include cardiac sarcoidosis (transcriptomic profiling of granuloma), lung adenocarcinoma (immunogenomic analysis of tumor antigens), and renal cell carcinoma (plasma proteomics-based drug resistance prediction). Applications cited include IF and IHC.
| Species | Application | Title |
|---|---|---|
Comput Struct Biotechnol J Integrated immunogenomic analysis of single-cell and bulk profiling reveals novel tumor antigens and subtype-specific therapeutic agents in lung adenocarcinoma | ||
Transl Androl Urol A prediction model for targeted drug resistance in renal cell carcinoma based on plasma proteomics analysis. |
