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CHURC1 Polyclonal antibody

CHURC1 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 12247-1-AP

Synonyms

C14orf52, chch, CHURC1, churchill domain containing 1, My015, Protein Churchill

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse skin tissue using CHURC1 Polyclonal antibody (12247-1-AP)

    WB analysis of mouse skin using 12247-1-AP

    mouse skin tissue were subjected to SDS PAGE followed by western blot with 12247-1-AP (CHURC1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using CHURC1 Polyclonal antibody (12247-1-AP)

    WB analysis of mouse liver using 12247-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 12247-1-AP (CHURC1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using CHURC1 Polyclonal antibody (12247-1-AP)

    WB analysis of HeLa using 12247-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 12247-1-AP (CHURC1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using CHURC1 Polyclonal antibody (12247-1-AP)

    WB analysis of mouse liver using 12247-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 12247-1-AP (CHURC1 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of U-937 cells using CHURC1 Polyclonal antibody (12247-1-AP)

    WB analysis of U-937 using 12247-1-AP

    U-937 cells were subjected to SDS PAGE followed by western blot with 12247-1-AP (CHURC1 antibody) at dilution of 1:50 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human brain tissue using CHURC1 Polyclonal antibody (12247-1-AP)

    IHC staining of human brain using 12247-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 12247-1-AP (CHURC1 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using CHURC1 Polyclonal antibody (12247-1-AP)

    IHC staining of human brain using 12247-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 12247-1-AP (CHURC1 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human gliomas tissue using CHURC1 Polyclonal antibody (12247-1-AP)

    IHC staining of human gliomas using 12247-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12247-1-AP (CHURC1 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human gliomas tissue using CHURC1 Polyclonal antibody (12247-1-AP)

    IHC staining of human gliomas using 12247-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12247-1-AP (CHURC1 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using CHURC1 Polyclonal antibody (12247-1-AP)

    IHC staining of human kidney using 12247-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12247-1-AP (CHURC1 Antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inmouse skin tissue, HeLa cells, mouse liver tissue
Positive IHC detected inhuman brain tissue, human gliomas tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12247-1-AP targets CHURC1 in WB, IHC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CHURC1 fusion protein Ag2892
Full Name churchill domain containing 1
Calculated Molecular Weight 13 kDa
Observed Molecular Weight 13 kDa
GenBank Accession NumberBC020550
Gene Symbol CHURC1
Gene ID (NCBI) 91612
RRIDAB_10666204
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CHURC1 (churchill domain containing 1), also known as C14orf52 or chch. The function of CHURC1 has not been widely studied, and is yet to be fully elucidated. It may be a transcriptional activator that mediates FGF signaling during neural development.The MW of this protein is 13 kDa, and Catalog#12247-1-AP specially recognises the 13 kDa protein.

Protocols

Product Specific Protocols
WB protocol for CHURC1 antibody 12247-1-APDownload protocol
IHC protocol for CHURC1 antibody 12247-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols