Claudin 18 Antibody

Claudin 18 Monoclonal Antibody for IHC, IP, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

IP, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

5G7F2

Cat no : 66167-1-Ig

Synonyms

claudin 18, CLDN18, SFTA5, SFTPJ



Tested Applications

Positive IP detected inmouse stomach tissue
Positive IHC detected inhuman stomach tissue, human pancreas cancer tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Product Information

66167-1-Ig targets Claudin 18 in IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Claudin 18 fusion protein Ag15517
Full Name claudin 18
Calculated molecular weight 261aa,28 kDa
Observed molecular weight 29 kDa
GenBank accession number BC146668
Gene symbol CLDN18
Gene ID (NCBI) 51208
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Claudin 18 (CLDN18) belongs to the large claudin family of proteins, which are tetraspan transmembrane proteins of tight junctions (PMID: 11585919; 18036336). Claudins exhibit specific patterns of expression in different tissues (PMID: 15447685). CLDN18 is specifically expressed in the stomach and lung. CLDN18 has two alternatively spliced variants, CLDN18.1 and CLDN18.2. CLDN18.2 is a highly selective gastric lineage marker that determines the gastric phenotype in a neoplastic condition, whereas CLDN18.1 is lung specific (PMID: 11585919; 21832145). Altered CLDN18 expression has been reported in various human malignancies, including gastric cancer, lung adenocarcinoma, and pancreatic neoplasm (PMID: 17459057; 22076167; 21832145).

Protocols

Product Specific Protocols
IHC protocol for Claudin 18 antibody 66167-1-IgDownload protocol
IP protocol for Claudin 18 antibody 66167-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

World J Gastrointest Pathophysiol

Visualization of sphingolipids and phospholipids in the fundic gland mucosa of human stomach using imaging mass spectrometry.

Authors - Nobuya Kurabe