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CLTB Polyclonal antibody

CLTB Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Cat no : 10455-1-AP

Synonyms

Clathrin light chain B, clathrin, light chain (Lcb), CLTB, LCB



Tested Applications

Positive WB detected inmouse thymus tissue, human brain tissue, mouse brain tissue, PC-3 cells
Positive IP detected inmouse brain tissue
Positive IF detected inHeLa cells, HEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10455-1-AP targets CLTB in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CLTB fusion protein Ag0707
Full Name clathrin, light chain (Lcb)
Calculated molecular weight 23 kDa
Observed molecular weight 32-38 kDa
GenBank accession numberBC006457
Gene symbol CLTB
Gene ID (NCBI) 1212
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Clathrin is the major protein of the polyhedral coat of coated pits and vesicles which entrap specific macromolecules during receptor-mediated endocytosis. The clathrin molecule has a triskelion shape. Each clathrin triskelion is composed of three identical heavy chains (180 kDa) and three light chains of two types, LCA (CLTA) and LCB (CLTB) (30-40 kDa). The light chain subunits are thought to regulate the formation or disassembly of clathrin coats. (PMID: 2445759; 8374173; 3563513)

Protocols

Product Specific Protocols
WB protocol for CLTB antibody 10455-1-APDownload protocol
IF protocol for CLTB antibody 10455-1-APDownload protocol
IP protocol for CLTB antibody 10455-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Proc Natl Acad Sci U S A

Clathrin light chains' role in selective endocytosis influences antibody isotype switching.

Authors - Shuang Wu
  • KD Validated