Filter: AllWBIPIHCIF/ICC

WB analysis using 12393-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12393-1-AP (COPG antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
WB analysis of A375 using 12393-1-AP
A375 cells were subjected to SDS PAGE followed by western blot with 12393-1-AP (COPG antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human colon using 12393-1-AP
human colon tissue were subjected to SDS PAGE followed by western blot with 12393-1-AP (COPG antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 12393-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 12393-1-AP (COPG antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 12393-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 12393-1-AP (COPG antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of K-562 using 12393-1-AP
IP result of anti-COPG (IP:12393-1-AP, 4ug; Detection:12393-1-AP 1:2000) with K-562 cells lysate 3600ug.
IHC staining of mouse kidney using 12393-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 12393-1-AP (COPG antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse kidney using 12393-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 12393-1-AP (COPG antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 12393-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer using 12393-1-AP (COPG antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human breast cancer using 12393-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12393-1-AP (COPG Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human breast cancer using 12393-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12393-1-AP (COPG Antibody) at dilution of 1:200 (under 40x lens).
IF Staining of HepG2 using 12393-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using COPG antibody (12393-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
IF Staining of HepG2 using 12393-1-AP
Immunofluorescent analysis of HepG2 cells, using COPG antibody 12393-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
IF Staining of NIH/3T3 using 12393-1-AP
Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using COPG antibody (12393-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
IF Staining of HeLa using 12393-1-AP
Immunofluorescent analysis of Hela cells, using COPG antibody 12393-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

COPG Polyclonal antibody

COPG Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 12393-1-AP

Publications(7)

Reviews (3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

Gamma-1-COP, Gamma-1-coat protein, COPG1, Coatomer subunit gamma-1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Publications (7)
Protocols
Reviews (3)

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	K-562 cells, A375 cells, HeLa cells, human brain tissue, human colon tissue, NIH/3T3 cells
Positive IP detected in	K-562 cells
Positive IHC detected in	mouse kidney tissue, human breast cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells, HeLa cells, NIH/3T3 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:500-1:2000
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 5 publications below
IF	See 3 publications below
IP	See 1 publications below

Product Information

12393-1-AP targets COPG in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag3070 Product name: Recombinant human COPG protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 350-699 aa of BC020498 Sequence: RDRATFYLNVLEQKQKALNAGYILNGLTVSIPGLERALQQYTLEPSEKPFDLKSVPLATAPMAEQRTESTPITAVKQPEKVAATRQEIFQEQLAAVPEFRGLGPLFKSSPEPVALTESETEYVIRCTKHTFTNHMVFQFDCTNTLNDQTLENVTVQMEPTEAYEVLCYVPARSLPYNQPGTCYTLVALPKEDPTAVACTFSCMMKFTVKDCDPTTGETDDEGYEDEYVLEDLEVTVADHIQKVMKLNFEAAWDEVGDEFEKEETFTLSTIKTLEEAVGNIVKFLGMHPCERSDKVPDNKNTHTLLLAGVFRGGHDILVRSRLLLLDTVTMQVTARSLEELPVDIILASVG Predict reactive species
Full Name	coatomer protein complex, subunit gamma
Calculated Molecular Weight	874 aa, 98 kDa
Observed Molecular Weight	98 kDa
GenBank Accession Number	BC020498
Gene Symbol	COPG
Gene ID (NCBI)	22820
RRID	AB_2081435
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9Y678
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IF protocol for COPG antibody 12393-1-AP	Download protocol
IHC protocol for COPG antibody 12393-1-AP	Download protocol
IP protocol for COPG antibody 12393-1-AP	Download protocol
WB protocol for COPG antibody 12393-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IF	Nat Commun Deficiency in coatomer complex I causes aberrant activation of STING signalling. Authors - Annemarie Steiner View Article
	IF	J Exp Med Trapping of CDC42 C-terminal variants in the Golgi drives pyrin inflammasome hyperactivation. Authors - Masahiko Nishitani-Isa View Article
mouse	WB	J Biol Chem Hepatic loss ofLissencephaly 1induces fatty liver and accelerates liver tumorigenesis in mice. Authors - Xiaoling Li View Article
mouse	WB	Hum Mol Genet Quantitative proteomics identifies proteins that resist translational repression and become dysregulated in ALS-FUS. Authors - Desiree M Baron View Article
human	IP,IF,WB	Sci Adv HPV is a cargo for the COPI sorting complex during virus entry Authors - Mara C Harwood View Article
human	WB	Adv Sci (Weinh) Targeting COPA to Enhance Erdafitinib Sensitivity in FGFR-Altered Bladder Cancer Authors - Huayuan Zhao View Article

Reviews

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The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH RICHA (Verified Customer) (03-02-2022)	Great antibody for IF Applications: Western Blot, Immunofluorescence Primary Antibody Dilution: 1:1000 Cell Tissue Type: Mouse tendon fibroblast
FH RICHA (Verified Customer) (11-16-2020)	Excellent antibody Applications: Western Blot, Immunofluorescence Primary Antibody Dilution: 1:500 Cell Tissue Type: Mouse fibroblast
FH RICHA (Verified Customer) (12-02-2019)	Excellent, no background Applications: Immunofluorescence, Primary Antibody Dilution: 1:500 Cell Tissue Type: Mouse fibroblast