COPG Polyclonal antibody

COPG Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 12393-1-AP

Synonyms

Coatomer subunit gamma, COPG, COPG1, Gamma coat protein, Gamma COP



Tested Applications

Positive WB detected inK-562 cells, A375 cells, HeLa cells, human brain tissue, human colon tissue, NIH/3T3 cells
Positive IP detected inK-562 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, Hela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 2 publications below

Product Information

12393-1-AP targets COPG in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen COPG fusion protein Ag3070
Full Name coatomer protein complex, subunit gamma
Calculated molecular weight 874 aa, 98 kDa
Observed molecular weight 98 kDa
GenBank accession numberBC020498
Gene symbol COPG
Gene ID (NCBI) 22820
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for COPG antibody 12393-1-APDownload protocol
IHC protocol for COPG antibody 12393-1-APDownload protocol
IF protocol for COPG antibody 12393-1-APDownload protocol
IP protocol for COPG antibody 12393-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Hum Mol Genet

Quantitative proteomics identifies proteins that resist translational repression and become dysregulated in ALS-FUS.

Authors - Desiree M Baron
mouseWB

J Biol Chem

Hepatic loss ofLissencephaly 1induces fatty liver and accelerates liver tumorigenesis in mice.

Authors - Xiaoling Li

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

RICHA (Verified Customer) (12-02-2019)

Excellent, no background

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Mouse fibroblast