Tested Applications
| Positive WB detected in | mouse skeletal muscle tissue, rat skeletal muscle tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
86557-1-RR targets COQ10A in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag12126 Product name: Recombinant human COQ10A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-247 aa of BC073923 Sequence: MAWAGSRRVPAGTRAAAERCCRLSLSPGAQPAPPPGPLPPPRPMRFLTSCSLLLPRAAQILAAEAGLPSSRSFMGFAAPFTNKRKAYSERRIMGYSMQEMYEVVSNVQEYREFVPWCKKSLVVSSRKGHLKAQLEVGFPPVMERYTSAVSMVKPHMVKAVCTDGKLFNHLETIWRFSPGIPAYPRTCTVDFSISFEFRSLLHSQLATMFFDEVVKQNVAAFERRAATKFGPETAIPRELMFHEVHQT Predict reactive species |
| Full Name | coenzyme Q10 homolog A (S. cerevisiae) |
| Calculated Molecular Weight | 247 aa, 28 kDa |
| Observed Molecular Weight | 20 kDa |
| GenBank Accession Number | BC073923 |
| Gene Symbol | COQ10A |
| Gene ID (NCBI) | 93058 |
| RRID | AB_3744966 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q96MF6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
COQ10A is a human gene that encodes a protein involved in the biosynthesis of coenzyme Q10 (CoQ10), also known as ubiquinone. CoQ10 is a lipid-soluble antioxidant and a critical component of the mitochondrial electron transport chain, where it plays a key role in cellular energy production (ATP synthesis). Mutations or deficiencies in COQ10A have been implicated in primary CoQ10 deficiency syndromes, which can manifest as mitochondrial disorders such as encephalomyopathy, nephropathy, and Leigh syndrome.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for COQ10A antibody 86557-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
