• Featured Product
  • KD/KO Validated

COTL1 Polyclonal antibody

COTL1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 10781-1-AP

Synonyms

CLP, Coactosin like protein, COTL1



Tested Applications

Positive WB detected inmouse brain tissue, HEK-293 cells, HeLa cells, human brain tissue, human kidney tissue, human placenta tissue, mouse placenta tissue, rat brain tissue
Positive IP detected inmouse kidney tissue
Positive IHC detected inhuman lung cancer tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:1000-1:6000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

10781-1-AP targets COTL1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen COTL1 fusion protein Ag1230
Full Name coactosin-like 1 (Dictyostelium)
Calculated molecular weight 16 kDa
Observed molecular weight 16 kDa
GenBank accession numberBC010884
Gene symbol COTL1
Gene ID (NCBI) 23406
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

COTL1, also named as CLP, binds to F-actin in a calcium-independent manner. And it has no direct effect on actin depolymerization.

Protocols

Product Specific Protocols
WB protocol for COTL1 antibody 10781-1-APDownload protocol
IHC protocol for COTL1 antibody 10781-1-APDownload protocol
IF protocol for COTL1 antibody 10781-1-APDownload protocol
IP protocol for COTL1 antibody 10781-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Biochim Biophys Acta

Stabilisation and characterisation of the isolated regulatory domain of human 5-lipoxygenase.

Authors - Mirjam Schröder
humanWB

Exp Lung Res

Exposure to cigarette smoke extract and lipopolysaccharide modifies cytoskeleton organization in bronchial epithelial cells.

Authors - Claudia D'Anna
humanWB

J Investig Med

Differential expression of PAI-RBP1, C1orf142, and COTL1 in non-small cell lung cancer cell lines with different tumor metastatic potential.

Authors - Sun Wenjing W
humanWB

PLoS One

TDP-43 identified from a genome wide RNAi screen for SOD1 regulators.

Authors - Somalinga Balajee R BR
  • KD Validated
humanWB

Oncotarget

Genetic and epigenetic silencing of mircoRNA-506-3p enhances COTL1 oncogene expression to foster non-small lung cancer progression.

Authors - Shanqi Guo
humanWB,IHC

J Proteome Res

Proteomic analysis of human small cell lung cancer tissues: up-regulation of coactosin-like protein-1.

Authors - Jeong Hye-Cheol HC

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Susanne (Verified Customer) (06-29-2019)

Good staining with low background in WB and IF. For IF fixation was done with methanol 10 min at -20°C (pre cool), the antibody was diluted in 1%BSA/PBS. For WB the antibody was diluted in PBST-0,1%/5%skim milk and incubated over night.

  • Applications: Western Blot,Immunofluorescence,
  • Primary Antibody Dilution: 1:1000 WB 1:50 IF
  • Cell Tissue Type: EBV transformed B cells, CD19+ cells, CD40L/IL-4 stimulated cells
COTL1 Antibody Western Blot,Immunofluorescence, validation (1:1000 WB 1:50 IF dilution) in EBV transformed B cells, CD19+ cells, CD40L/IL-4 stimulated cells (Cat no:10781-1-AP)