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- KD/KO Validated
COTL1 Polyclonal antibody
COTL1 Polyclonal Antibody for IF, IHC, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, IF,ELISA
Conjugate
Unconjugated
Cat no : 10781-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse brain tissue, HEK-293 cells, HeLa cells, human brain tissue, human kidney tissue, human placenta tissue, mouse placenta tissue, rat brain tissue |
| Positive IP detected in | mouse kidney tissue |
| Positive IHC detected in | human lung cancer tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:1000-1:6000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:20-1:200 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 6 publications below |
| IHC | See 1 publications below |
Product Information
10781-1-AP targets COTL1 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | COTL1 fusion protein Ag1230 |
| Full Name | coactosin-like 1 (Dictyostelium) |
| Calculated molecular weight | 16 kDa |
| Observed molecular weight | 16 kDa |
| GenBank accession number | BC010884 |
| Gene symbol | COTL1 |
| Gene ID (NCBI) | 23406 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
COTL1, also named as CLP, binds to F-actin in a calcium-independent manner. And it has no direct effect on actin depolymerization.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for COTL1 antibody 10781-1-AP | Download protocol |
| IHC protocol for COTL1 antibody 10781-1-AP | Download protocol |
| IF protocol for COTL1 antibody 10781-1-AP | Download protocol |
| IP protocol for COTL1 antibody 10781-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Biochim Biophys Acta Stabilisation and characterisation of the isolated regulatory domain of human 5-lipoxygenase. | ||
Exp Lung Res Exposure to cigarette smoke extract and lipopolysaccharide modifies cytoskeleton organization in bronchial epithelial cells. | ||
J Investig Med Differential expression of PAI-RBP1, C1orf142, and COTL1 in non-small cell lung cancer cell lines with different tumor metastatic potential. | ||
PLoS One TDP-43 identified from a genome wide RNAi screen for SOD1 regulators.
| ||
Oncotarget Genetic and epigenetic silencing of mircoRNA-506-3p enhances COTL1 oncogene expression to foster non-small lung cancer progression. | ||
J Proteome Res Proteomic analysis of human small cell lung cancer tissues: up-regulation of coactosin-like protein-1. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Susanne (Verified Customer) (06-29-2019) | Good staining with low background in WB and IF.For IF fixation was done with methanol 10 min at -20°C (pre cool), the antibody was diluted in 1%BSA/PBS.For WB the antibody was diluted in PBST-0,1%/5%skim milk and incubated over night.
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