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COTL1 Antibody 6 Publications

Rabbit Polyclonal| Catalog number: 10781-1-AP

Featured Product KD/KO validated

1 customer review

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Con: 52 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, ELISA
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COTL1 Antibody IHC human lung cancer tissue 10781-1-AP

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10781-1-AP( COTL1 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

COTL1 Antibody IHC human lung cancer tissue 10781-1-AP

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10781-1-AP( COTL1 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

COTL1 Antibody WB mouse brain tissue 10781-1-AP

mouse brain tissue were subjected to SDS PAGE followed by western blot with 10781-1-AP(COTL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

COTL1 Antibody IP mouse kidney tissue 10781-1-AP

IP Result of anti-COTL1 (IP:10781-1-AP, 3ug; Detection:10781-1-AP 1:3000) with mouse kidney tissue lysate 12500ug.

COTL1 Antibody IF HepG2 cells 10781-1-AP

Immunofluorescent analysis of HepG2 cells, using COTL1 antibody 10781-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Species specificity:
human, mouse, rat
Positive WB detected in:
mouse brain tissue, HEK-293 cells, HeLa cells, human brain tissue, human kidney tissue, human placenta tissue, mouse placenta tissue, rat brain tissue
Positive IP detected in:
mouse kidney tissue
Positive IHC detected in:
human lung cancer tissue, human kidney tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HepG2 cells
Recommended dilution:
WB : 1:500-1:2000
IP : 0.5-4.0 ug for IP and 1:1000-1:6000 for WB
IHC : 1:50-1:500
IF : 1:20-1:200
Sample-dependent, check data in Validation tab

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
COTL1 fusion protein Ag1230
Full name:
coactosin-like 1 (Dictyostelium)
Calculated molecular weight:
16 kDa
Observed molecular weight:
16 kDa
GenBank accession number:
BC010884
Gene ID (NCBI):
23406
Gene symbol
COTL1
Synonyms
CLP, Coactosin like protein, COTL1

Background

COTL1, also named as CLP, binds to F-actin in a calcium-independent manner. And it has no direct effect on actin depolymerization.

Customer Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

Share your scientific insight on this antibody

COTL1 Rabbit Polyclonal antibody

Susanne (Verified Customer), 06-29-2019
Applications
Western Blot,Immunofluorescence,
Primary Antibody Dilution
1:1000 WB 1:50 IF
COTL1 Antibody Western Blot,Immunofluorescence, validation (1:1000 WB 1:50 IF dilution) in EBV transformed B cells, CD19+ cells, CD40L/IL-4 stimulated cells (Cat no:10781-1-AP)

COTL1 Antibody Western Blot,Immunofluorescence, validation (1:1000 WB 1:50 IF dilution) in EBV transformed B cells, CD19+ cells, CD40L/IL-4 stimulated cells (Cat no:10781-1-AP)

Cell/Tissue Type
EBV transformed B cells, CD19+ cells, CD40L/IL-4 stimulated cells
Lot Number
Good staining with low background in WB and IF. For IF fixation was done with methanol 10 min at -20°C (pre cool), the antibody was diluted in 1%BSA/PBS. For WB the antibody was diluted in PBST-0,1%/5%skim milk and incubated over night.

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