Tested Applications
| Positive WB detected in | A549 cells, HeLa cells, mouse brain tissue, rat brain tissue |
| Positive IP detected in | mouse kidney tissue |
| Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 9 publications below |
| IHC | See 4 publications below |
| IF | See 1 publications below |
Product Information
10781-1-AP targets COTL1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | COTL1 fusion protein Ag1230 Predict reactive species |
| Full Name | coactosin-like 1 (Dictyostelium) |
| Calculated Molecular Weight | 16 kDa |
| Observed Molecular Weight | 16 kDa |
| GenBank Accession Number | BC010884 |
| Gene Symbol | COTL1 |
| Gene ID (NCBI) | 23406 |
| RRID | AB_2084785 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q14019 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
COTL1, also named as CLP, binds to F-actin in a calcium-independent manner. And it has no direct effect on actin depolymerization.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for COTL1 antibody 10781-1-AP | Download protocol |
| IHC protocol for COTL1 antibody 10781-1-AP | Download protocol |
| IP protocol for COTL1 antibody 10781-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Proteome Res Proteomic analysis of human small cell lung cancer tissues: up-regulation of coactosin-like protein-1. | ||
Oncotarget Genetic and epigenetic silencing of mircoRNA-506-3p enhances COTL1 oncogene expression to foster non-small lung cancer progression. | ||
PLoS One TDP-43 identified from a genome wide RNAi screen for SOD1 regulators.
| ||
Exp Lung Res Exposure to cigarette smoke extract and lipopolysaccharide modifies cytoskeleton organization in bronchial epithelial cells. | ||
J Investig Med Differential expression of PAI-RBP1, C1orf142, and COTL1 in non-small cell lung cancer cell lines with different tumor metastatic potential. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Susanne (Verified Customer) (06-29-2019) | Good staining with low background in WB and IF.For IF fixation was done with methanol 10 min at -20°C (pre cool), the antibody was diluted in 1%BSA/PBS.For WB the antibody was diluted in PBST-0,1%/5%skim milk and incubated over night.
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