|Positive WB detected in||mouse brain tissue, HEK-293 cells, HeLa cells, human brain tissue, human kidney tissue, human placenta tissue, mouse placenta tissue, rat brain tissue|
|Positive IP detected in||mouse kidney tissue|
|Positive IHC detected in||human lung cancer tissue, human kidney tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:1000-1:6000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
10781-1-AP targets COTL1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||COTL1 fusion protein Ag1230|
|Full Name||coactosin-like 1 (Dictyostelium)|
|Calculated molecular weight||16 kDa|
|Observed molecular weight||16 kDa|
|GenBank accession number||BC010884|
|Gene ID (NCBI)||23406|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
COTL1, also named as CLP, binds to F-actin in a calcium-independent manner. And it has no direct effect on actin depolymerization.
|Product Specific Protocols|
|WB protocol for COTL1 antibody 10781-1-AP||Download protocol|
|IHC protocol for COTL1 antibody 10781-1-AP||Download protocol|
|IF protocol for COTL1 antibody 10781-1-AP||Download protocol|
|IP protocol for COTL1 antibody 10781-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Biochim Biophys Acta
Stabilisation and characterisation of the isolated regulatory domain of human 5-lipoxygenase.
Exp Lung Res
Exposure to cigarette smoke extract and lipopolysaccharide modifies cytoskeleton organization in bronchial epithelial cells.
J Investig Med
Differential expression of PAI-RBP1, C1orf142, and COTL1 in non-small cell lung cancer cell lines with different tumor metastatic potential.
TDP-43 identified from a genome wide RNAi screen for SOD1 regulators.
Genetic and epigenetic silencing of mircoRNA-506-3p enhances COTL1 oncogene expression to foster non-small lung cancer progression.
J Proteome Res
Proteomic analysis of human small cell lung cancer tissues: up-regulation of coactosin-like protein-1.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Susanne (Verified Customer) (06-29-2019)
Good staining with low background in WB and IF. For IF fixation was done with methanol 10 min at -20°C (pre cool), the antibody was diluted in 1%BSA/PBS. For WB the antibody was diluted in PBST-0,1%/5%skim milk and incubated over night.