COX2/ Cyclooxygenase 2/ PTGS2 Monoclonal antibody
COX2/ Cyclooxygenase 2/ PTGS2 Monoclonal Antibody for IF, IHC, WB,ELISA
Cat no : 66351-1-Ig
|Positive WB detected in||LPS treated HeLa cells, LPS treated RAW 264.7 cells|
|Positive IHC detected in||human lung cancer tissue, human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||mouse lung tissue|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunohistochemistry (IHC)||IHC : 1:100-1:400|
|Immunofluorescence (IF)||IF : 1:200-1:800|
|Sample-dependent, check data in validation data gallery|
66351-1-Ig targets COX2/ Cyclooxygenase 2/ PTGS2 in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||COX2/ Cyclooxygenase 2/ PTGS2 fusion protein Ag24721|
|Full Name||prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase)|
|Calculated molecular weight||604 aa, 68 kDa|
|Observed molecular weight||66-70 kDa|
|GenBank accession number||BC013734|
|Gene ID (NCBI)||5743|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
COX2 (Prostaglandin G/H synthase 2, PTGS2) mediates the formation of prostaglandins from arachidonate. Its subunit structure is homodimer. The fully N-glycosylated PTGS2 is 72-74 kDa and the aglycosylated is 66 kDa (PMID:19656660). It also expresses a band of 39 kDa after unspecific cleavage (PMID:17509125). The 50 kDa band of fragmented PTGS2 has also previously been detected in AD brains (PMID:14724276).
|Product Specific Protocols|
|WB protocol for COX2/ Cyclooxygenase 2/ PTGS2 antibody 66351-1-Ig||Download protocol|
|IHC protocol for COX2/ Cyclooxygenase 2/ PTGS2 antibody 66351-1-Ig||Download protocol|
|IF protocol for COX2/ Cyclooxygenase 2/ PTGS2 antibody 66351-1-Ig||Download protocol|
|Click here to view our Standard Protocols|
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