COX2/ Cyclooxygenase 2/ PTGS2 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

COX2/ Cyclooxygenase 2/ PTGS2 Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

3G2B9

Cat no : 66351-1-PBS

Synonyms

COX 2, COX2, COX2/ Cyclooxygenase 2, COX2/ Cyclooxygenase 2/ PTGS2, Cyclooxygenase 2, GRIPGHS, hCox 2, PGG/HS, PGH synthase 2, PGHS 2, PHS 2, PHS II, Prostaglandin G/H synthase 2, Prostaglandin H2 synthase 2, PTGS2

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Validation Data Gallery

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  WB analysis using 66351-1-Ig (same clone as 66351-1-PBS)

  Non-treated and LPS treated RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66351-1-Ig (COX2/ Cyclooxygenase 2/ PTGS2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66351-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66351-1-Ig (same clone as 66351-1-PBS)

  WB result of COX2/ Cyclooxygenase 2/ PTGS2 antibody (66351-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-control and sh-COX2/ Cyclooxygenase 2/ PTGS2 transfected HEK-293 cells. This data was developed using the same antibody clone with 66351-1-PBS in a different storage buffer formulation.

• 

  WB analysis of RAW 264.7 using 66351-1-Ig (same clone as 66351-1-PBS)

  Untreated and LPS-treated RAW264.7 cells were subjected to SDS PAGE followed by western blot with 66351-1-Ig (COX2/ Cyclooxygenase 2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66351-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 66351-1-Ig (same clone as 66351-1-PBS)

  Untreated and LPS-treated Hela cells were subjected to SDS PAGE followed by western blot with 66351-1-Ig (COX2/ Cyclooxygenase 2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66351-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 66351-1-Ig (same clone as 66351-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66351-1-Ig (COX2/ Cyclooxygenase 2/ PTGS2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66351-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66351-1-Ig (same clone as 66351-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66351-1-Ig (COX2/ Cyclooxygenase 2/ PTGS2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66351-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66351-1-Ig (same clone as 66351-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66351-1-Ig (COX2/ Cyclooxygenase 2/ PTGS2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66351-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66351-1-Ig (same clone as 66351-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66351-1-Ig (COX2/ Cyclooxygenase 2/ PTGS2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66351-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse lung using 66351-1-Ig (same clone as 66351-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse lung tissue using COX2/ Cyclooxygenase 2/ PTGS2 antibody (66351-1-Ig, Clone: 3G2B9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66351-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse lung using 66351-1-Ig (same clone as 66351-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse lung tissue using COX2/ Cyclooxygenase 2/ PTGS2 antibody (66351-1-Ig, Clone: 3G2B9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66351-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66351-1-PBS targets COX2/ Cyclooxygenase 2/ PTGS2 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: COX2/ Cyclooxygenase 2/ PTGS2 fusion protein Ag24721
• Full Name: prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase)
• Calculated Molecular Weight: 604 aa, 68 kDa
• Observed Molecular Weight: 66-70 kDa
• GenBank Accession Number: BC013734
• Gene Symbol: PTGS2
• Gene ID (NCBI): 5743
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -20°C. Stable for one year after shipment.

Background Information

COX2 (Prostaglandin G/H synthase 2, PTGS2) mediates the formation of prostaglandins from arachidonate. Its subunit structure is homodimer. The fully N-glycosylated PTGS2 is 72-74 kDa and the aglycosylated is 66 kDa (PMID:19656660). It also expresses a band of 39 kDa after unspecific cleavage (PMID:17509125). The 50 kDa band of fragmented PTGS2 has also previously been detected in AD brains (PMID:14724276).