|Positive WB detected in||HeLa cells|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human colon cancer tissue, human skin tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
10225-1-AP targets CRABP2 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||CRABP2 fusion protein Ag0309|
|Full Name||cellular retinoic acid binding protein 2|
|Calculated molecular weight||16 kDa|
|Observed molecular weight||16 kDa|
|GenBank accession number||BC001109|
|Gene ID (NCBI)||1382|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.1% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Cellular retinoic acid binding protein 2 (CRABP2, synonyms: RBP6, CRABP-II). A number of specific carrier proteins for members of the vitamin A family have been discovered. Cellular retinoic acid binding proteins (CRABP) are low molecular weight proteins whose precise function remains unknown. CRABP2 is important in retinoic acid-mediated regulation of human skin growth and differentiation. It has been postulated that the CRABP2 gene is transcriptionally regulated by a newly synthesized regulatory protein.
Crabp2 Promotes Metastasis of Lung Cancer Cells via HuR and Integrin β1/FAK/ERK Signaling.
J Exp Clin Cancer Res
CRABP2 regulates invasion and metastasis of breast cancer through hippo pathway dependent on ER status.
Hes1 and Hes5 are required for differentiation of pituicytes and formation of the neurohypophysis in pituitary development.
Front Endocrinol (Lausanne)
Resveratrol Reverses Retinoic Acid Resistance of Anaplastic Thyroid Cancer Cells via Demethylating CRABP2 Gene.
Col1a1+ perivascular cells in the brain are a source of retinoic acid following stroke.
Histochem Cell Biol
Retinoic acid regulates cell-shape and -death of E-FABP (FABP5)-immunoreactive septoclasts in the growth plate cartilage of mice.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Yan (Verified Customer) (01-27-2020)
I used this antibody on mouse embryonic section for the immunofluorescence. It worked very well.
lunfeng (Verified Customer) (01-27-2020)
Jie (Verified Customer) (01-27-2020)
VERY GOOD ANTIBODY. WORKS IN KO VERSUS WT CONTROL MICE.