• Featured Product
  • KD/KO Validated

CRABP2 Polyclonal antibody

CRABP2 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 10225-1-AP

Synonyms

CRABP II, CRABP2, RBP6



Tested Applications

Positive WB detected inHeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman colon cancer tissue, human skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

10225-1-AP targets CRABP2 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CRABP2 fusion protein Ag0309
Full Name cellular retinoic acid binding protein 2
Calculated molecular weight 16 kDa
Observed molecular weight 16 kDa
GenBank accession numberBC001109
Gene symbol CRABP2
Gene ID (NCBI) 1382
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cellular retinoic acid binding protein 2 (CRABP2, synonyms: RBP6, CRABP-II). A number of specific carrier proteins for members of the vitamin A family have been discovered. Cellular retinoic acid binding proteins (CRABP) are low molecular weight proteins whose precise function remains unknown. CRABP2 is important in retinoic acid-mediated regulation of human skin growth and differentiation. It has been postulated that the CRABP2 gene is transcriptionally regulated by a newly synthesized regulatory protein.

Protocols

Product Specific Protocols
WB protocol for CRABP2 antibody 10225-1-APDownload protocol
IHC protocol for CRABP2 antibody 10225-1-APDownload protocol
IP protocol for CRABP2 antibody 10225-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,IHC,IP

Sci Rep

Crabp2 Promotes Metastasis of Lung Cancer Cells via HuR and Integrin β1/FAK/ERK Signaling.

Authors - Jun-I Wu
  • KD Validated
humanCoIP

J Exp Clin Cancer Res

CRABP2 regulates invasion and metastasis of breast cancer through hippo pathway dependent on ER status.

Authors - Xuefei Feng
mouseIF

Brain Res

Hes1 and Hes5 are required for differentiation of pituicytes and formation of the neurohypophysis in pituitary development.

Authors - Masanori Goto
humanWB,IHC

Front Endocrinol (Lausanne)

Resveratrol Reverses Retinoic Acid Resistance of Anaplastic Thyroid Cancer Cells via Demethylating CRABP2 Gene.

Authors - Xin Liu
mouseIF

BMC Neurosci

Col1a1+ perivascular cells in the brain are a source of retinoic acid following stroke.

Authors - Kathleen K Kelly
mouseIF

Histochem Cell Biol

Retinoic acid regulates cell-shape and -death of E-FABP (FABP5)-immunoreactive septoclasts in the growth plate cartilage of mice.

Authors - Yasuhiko Bando

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Yan (Verified Customer) (01-27-2020)

I used this antibody on mouse embryonic section for the immunofluorescence. It worked very well.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: Mouse embryonic section
FH

lunfeng (Verified Customer) (01-27-2020)

VERY GOOD

  • Applications: Immunofluorescence,
FH

Jie (Verified Customer) (01-27-2020)

VERY GOOD ANTIBODY. WORKS IN KO VERSUS WT CONTROL MICE.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: mouse brain tissue