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CREB3 Antibody

CREB3 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, IF, chIP, ELISA

Conjugate

Unconjugated

Cat no : 11275-1-AP

Synonyms

CREB 3, CREB3, LUMAN, LZIP



Tested Applications

Positive WB detected inJurkat cells, HeLa cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

11275-1-AP targets CREB3 in WB, IP, IHC, IF, chIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CREB3 fusion protein Ag1823
Full Name cAMP responsive element binding protein 3
Calculated molecular weight 44 kDa
Observed molecular weight 40-64 kDa
GenBank accession number BC009402
Gene symbol CREB3
Gene ID (NCBI) 10488
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CREB3, also named as LZIP and Luman, belongs to the bZIP family and ATF subfamily. It is a transcription factors activated upon intramembrane proteolysis (RIP), binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AG][AG]-3'), a sequence present in many viral and cellular promoters. CREB3 binds to and requires HCFC1 as a coactivator. The activity and expression are suppressed when the HCFC1-CREB3 complex binds with CREBZF. Participates in LKN-1/CCL15-induced chemotaxis signaling. CREB3 is N-glycosylated and released by proteolysis. So the MW of CREB3 emirate 40kd to 64kd (PMID: 12138176).

Protocols

Product Specific Protocols
WB protocol for CREB3 antibody 11275-1-APDownload protocol
IHC protocol for CREB3 antibody 11275-1-APDownload protocol
IF protocol for CREB3 antibody 11275-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIP,WB

Biochim Biophys Acta

JAB1/CSN5 inhibits the activity of Luman/CREB3 by promoting its degradation.

Authors - Denboer Lisa M LM
humanWB

FEBS Lett

Regulation of the ER-bound transcription factor Luman/CREB3 in HEK293 cells.

Authors - Kentaro Oh-Hashi
humanWB,IF,IP,chIP

Front Mol Neurosci

LUMAN/CREB3 Plays a Dual Role in Stress Responses as a Cofactor of the Glucocorticoid Receptor and a Regulator of Secretion.

Authors - Jenna Penney