Tested Applications
| Positive WB detected in | Jurkat cells, HeLa cells |
| Positive IF/ICC detected in | U2OS cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 15 publications below |
| IHC | See 3 publications below |
| IF | See 3 publications below |
| IP | See 2 publications below |
| ChIP | See 3 publications below |
Product Information
11275-1-AP targets CREB3 in WB, IHC, IF/ICC, IP, ChIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1823 Product name: Recombinant human CREB3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-371 aa of BC009402 Sequence: MELELDAGDQDLLAFLLEESGDLGTAPDEAVRAPLDWALPLSEVPSDWEVDDLLCSLLSPPASLNILSSSNPCLVHHDHTYSLPRETVSMDLESESCRKEGTQMTPQHMEELAEQEIARLVLTDEEKSLLEKEGLILPETLPLTKTEEQILKRVRRKIRNKRSAQESRRKKKVYVGGLESRVLKYTAQNMELQNKVQLLEEQNLSLLDQLRKLQAMVIEISNKTSSSSTCILVLLVSFCLLLVPAIYSSDTRGSLPAEHGVLSRQLRALPSEDPYQLELPALQSEVPKDSTHQWLDGSDCVLQAPGNTSCLLHYMPQAPSAEPPLEWPFPDLFSEPLCRGPILPLQANLTRKGGWLPTGSPSVILQDRYSG Predict reactive species |
| Full Name | cAMP responsive element binding protein 3 |
| Calculated Molecular Weight | 44 kDa |
| Observed Molecular Weight | 40-64 kDa |
| GenBank Accession Number | BC009402 |
| Gene Symbol | CREB3 |
| Gene ID (NCBI) | 10488 |
| RRID | AB_10640533 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O43889 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CREB3, also named as LZIP and Luman, belongs to the bZIP family and ATF subfamily. It is a transcription factors activated upon intramembrane proteolysis (RIP), binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AG][AG]-3'), a sequence present in many viral and cellular promoters. CREB3 binds to and requires HCFC1 as a coactivator. The activity and expression are suppressed when the HCFC1-CREB3 complex binds with CREBZF. Participates in LKN-1/CCL15-induced chemotaxis signaling. CREB3 is N-glycosylated and released by proteolysis. So the MW of CREB3 emirate 40kd to 64kd (PMID: 12138176).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CREB3 antibody 11275-1-AP | Download protocol |
| IHC protocol for CREB3 antibody 11275-1-AP | Download protocol |
| WB protocol for CREB3 antibody 11275-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Sci Adv Standalone or combinatorial phenylbutyrate therapy shows excellent antiviral activity and mimics CREB3 silencing.
| ||
Nat Commun Golgi stress induces SIRT2 to counteract Shigella infection via defatty-acylation. | ||
Int J Mol Sci Comparative Analysis of CREB3 and CREB3L2 Protein Expression in HEK293 Cells. | ||
Cancers (Basel) miR766-3p and miR124-3p Dictate Drug Resistance and Clinical Outcome in HNSCC | ||
Front Mol Neurosci LUMAN/CREB3 Plays a Dual Role in Stress Responses as a Cofactor of the Glucocorticoid Receptor and a Regulator of Secretion. | ||
Carcinogenesis Small leucine zipper protein promotes the metastasis of castration-resistant prostate cancer through transcriptional regulation of matrix metalloproteinase-13. |
