Tested Applications
| Positive WB detected in | Jurkat cells, HEK-293 cells, HeLa cells, mouse testis tissue, rat testis tissue |
| Positive IP detected in | Jurkat cells |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11931-1-AP targets CSPP1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2521 Product name: Recombinant human CSPP1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-208 aa of BC029445 Sequence: MADNLDEFIEEQKARLAEDKAELESDPPYMEMKGKLSAKLSENSKILISMAKENIPPNSQQTRGSLGIDYGLSLPLGEDYERKKHKLKEELRQDYRRYLTQERLKLERNKEYNQFLRGKEESSEKFRQVEKSTEPKSQRNKKPIGQVKPDLTSQIQTSCENSEGPRKDVLTPSEAYEELLNQRRLEEDRYRQLDDEIELRNRRIIKSK Predict reactive species |
| Full Name | centrosome and spindle pole associated protein 1 |
| Calculated Molecular Weight | 141 kDa |
| Observed Molecular Weight | 141 kDa |
| GenBank Accession Number | BC029445 |
| Gene Symbol | CSPP1 |
| Gene ID (NCBI) | 79848 |
| RRID | AB_2087897 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q1MSJ5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CSPP1 interacts with centrosomes and microtubules and plays a role in cell-cycle progression and spindle organization. CSPP1 is required for their common C-terminal domain to interact with Nephrocystin 8 (NPHP8/RPGRIP1L) and to form a ternary complex with NPHP8 and NPHP4, and for ciliogenesis in hTERT-RPE1 cells in vitro and are expressed in ciliated renal, retinal, and respiratory cells in vivo
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CSPP1 antibody 11931-1-AP | Download protocol |
| IP protocol for CSPP1 antibody 11931-1-AP | Download protocol |
| WB protocol for CSPP1 antibody 11931-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-CSPP1 monoclonal antibody (Cat# 11931-1-AP) has accumulated 13 citations across journals including Science Advances, Methods in Cell Biology, British Journal of Cancer, American Journal of Human Genetics, Cell Reports, PLoS Biology, EMBO Reports, Molecular Biology of the Cell, PLoS ONE, and Cilia. Associated research fields include ciliopathies (Joubert syndrome), primary cilia biology, Hedgehog signaling, cell migration, mitotic spindle organization, centriolar satellite stability, and breast cancer subtyping.
| Species | Application | Title |
|---|---|---|
Methods Cell Biol Fixation methods and immunolabeling for cilia proteins in ciliary and extraciliary locations | ||
Br J Cancer Nuclear CSPP1 expression defined subtypes of basal-like breast cancer.
| ||
Am J Hum Genet Mutations in CSPP1 Cause Primary Cilia Abnormalities and Joubert Syndrome with or without Jeune Asphyxiating Thoracic Dystrophy. | ||
Cell Rep A CEP104-CSPP1 Complex Is Required for Formation of Primary Cilia Competent in Hedgehog Signaling.
|
