|Positive WB detected in||Jurkat cells, HEK-293 cells, HeLa cells, mouse testis tissue, rat testis tissue|
|Positive IF detected in||Hela cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
11931-1-AP targets CSPP1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||CSPP1 fusion protein Ag2521|
|Full Name||centrosome and spindle pole associated protein 1|
|Calculated molecular weight||141 kDa|
|Observed molecular weight||141 kDa|
|GenBank accession number||BC029445|
|Gene ID (NCBI)||79848|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
CSPP1 interacts with centrosomes and microtubules and plays a role in cell-cycle progression and spindle organization. CSPP1 is required for their common C-terminal domain to interact with Nephrocystin 8 (NPHP8/RPGRIP1L) and to form a ternary complex with NPHP8 and NPHP4, and for ciliogenesis in hTERT-RPE1 cells in vitro and are expressed in ciliated renal, retinal, and respiratory cells in vivo
Fixation methods can differentially affect ciliary protein immunolabeling.
Mol Biol Cell
The E3 ubiquitin ligase UBR5 regulates centriolar satellite stability and primary cilia.
CSPP-L Associates with the Desmosome of Polarized Epithelial Cells and Is Required for Normal Spheroid Formation.
Mol Biol Cell
CSPP is a ciliary protein interacting with Nephrocystin 8 and required for cilia formation.
Br J Cancer
Nuclear CSPP1 expression defined subtypes of basal-like breast cancer.
A CEP104-CSPP1 Complex Is Required for Formation of Primary Cilia Competent in Hedgehog Signaling.