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CTGF Polyclonal antibody

CTGF Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (6)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 23936-1-AP

Synonyms

CCN family member 2, CCN2, CTGF, HCS24, IBP 8, IGF binding protein 8, IGFBP 8, IGFBP8, NOV2



Tested Applications

Positive WB detected inmouse heart tissue, mouse skeletal muscle tissue
Positive IHC detected inhuman heart tissue, human gliomas tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 23936-1-AP is CTGF Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivity buffloa, dog, human, miniping, mouse, pig, rat, sheep
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CTGF fusion protein Ag21008
Full Name connective tissue growth factor
Calculated molecular weight 349 aa, 38 kDa
Observed molecular weight 38 kDa
GenBank accession numberBC087839
Gene symbol CTGF
Gene ID (NCBI) 1490
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CTGF, also known as CCN2 or connective tissue growth factor, is a member of the CCN family of matricellular proteins. CTGF, a cysteine-rich, matrix-associated, heparin-binding protein, is widely expressed in various human tissues and organs. CTGF has important roles in many biological processes, including cell adhesion, migration, proliferation, angiogenesis, skeletal development, and tissue wound repair, and is critically involved in fibrotic disease and several forms of cancers. In western blotting, there are three different forms of CTGF reported: monomeric forms at approximately 36-38 kDa, homodimeric forms at approximately 70 kDa, and lower molecular mass fragment forms.

Protocols

Product Specific Protocols
WB protocol for CTGF antibody 23936-1-APDownload protocol
IHC protocol for CTGF antibody 23936-1-APDownload protocol
IF protocol for CTGF antibody 23936-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB,IF

Exp Cell Res

CTGF regulates cyclic stretch-induced vascular smooth muscle cell proliferation via microRNA-19b-3p.

Authors - Wen-Bin Wang
mouseWB

Biomed Pharmacother

Citrus alkaline extracts prevent fibroblast senescence to ameliorate pulmonary fibrosis via activation of COX-2.

Authors - Fanchao Feng
humanWB

Cells

Myricetin Suppresses the Propagation of Hepatocellular Carcinoma via Down-Regulating Expression of YAP.

Authors - Minjing Li

J Endocrinol

SRT1720 retards renal fibrosis via inhibition of HIF1α /GLUT1 in diabetic nephropathy.

Authors - Weixia Han
humanIHC

EBioMedicine

Aspirin targets P4HA2 through inhibiting NF-κB and LMCD1-AS1/let-7g to inhibit tumour growth and collagen deposition in hepatocellular carcinoma.

Authors - Tianjiao Wang
human,mouseWB,IHC

Cells

Induction of Fibrosis and Autophagy in Kidney Cells by Vinyl Chloride.

Authors - Yung-Ho Hsu