Tested Applications
| Positive WB detected in | MG-63 cells, U2OS cells, HT-1080 cells, Calu-3 cells, U-251 cells, HepG2 cells, HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
82976-1-RR targets CTGF in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | connective tissue growth factor |
| Calculated Molecular Weight | 38 kDa |
| Observed Molecular Weight | 35 kDa |
| GenBank Accession Number | NM_001901 |
| Gene Symbol | CTGF |
| Gene ID (NCBI) | 1490 |
| RRID | AB_3670724 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P29279 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CTGF, also known as CCN2, is a member of the CCN family of matricellular proteins. CTGF, a cysteine-rich, matrix-associated, heparin-binding protein, is widely expressed in various human tissues and organs. CTGF has important roles in many biological processes, including cell adhesion, migration, proliferation, angiogenesis, skeletal development, and tissue wound repair, and is critically involved in fibrotic disease and several forms of cancers.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CTGF antibody 82976-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CTGF Recombinant monoclonal antibody (230324B12), catalog number 82976-1-RR, has 2 total citations. These appear in Acta Pharmacologica Sinica and iScience. The research fields cover hepatocyte proliferation via YAP signaling in cholestatic disease and oncogenic USP3-mediated hepatocellular carcinoma progression through K11/K48-specific deubiquitination and stabilization of YAP.
| Species | Application | Title |
|---|---|---|
Acta Pharmacol Sin Stevioside promotes hepatocyte proliferation via YAP signaling in cholestatic disease. | ||
iScience Oncogenic USP3 promotes hepatocellular carcinoma progression via K11/K48-specific deubiquitination and stabilization of YAP. |
Reviews
What customers say
The single review for this recombinant rabbit antibody (used in Western Blot at 1/1000 dilution on mesenchymal stem cells) rates it 3 out of 5. The reviewer noted the antibody detects the target clearly and quickly, but the band appeared at a lower molecular weight than expected. They attributed this discrepancy to their own cell type and protein extraction method rather than the antibody itself. Overall, the feedback suggests reliable detection with a caveat about the observed molecular weight.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Zahida (Verified Customer) (04-15-2025) | It shows up well and quickly, but it's not at the right molecular weight — it's lower. That could be due to my cells and the way I extract the proteins
|
