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  • KD/KO Validated

Gamma cystathionase Antibody

Gamma cystathionase Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 12217-1-AP

Synonyms

CSE, CTH, Cystathionine gamma lyase, Gamma cystathionase



Tested Applications

Positive WB detected inmouse kidney tissue, rat liver tissue, mouse heart tissue, rat heart tissue, HepG2 cells, HeLa cells, mouse liver tissue, rat kidney tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman liver cancer tissue, human breast cancer tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:100-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

12217-1-AP targets Gamma cystathionase in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, Rabbit, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Gamma cystathionase fusion protein Ag2872
Full Name cystathionase (cystathionine gamma-lyase)
Calculated molecular weight 405 aa, 45 kDa
Observed molecular weight 40-45 kDa
GenBank accession numberBC015807
Gene symbol CTH
Gene ID (NCBI) 1491
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CTH, also named as Gamma-cystathionase and CSE, belongs to the transsulfuration enzymes family. It catalyzes the last step in the transsulfuration pathway from methionine to cysteine. CTH converts two cysteine molecules to lanthionine and hydrogen sulfide. CTH can also accept homocysteine as substrate. It specificity depends on the levels of the endogenous substrates. CTH is the major H2S-producing enzyme in kidney, liver, vascular smooth muscle cells and enterocytes. The endogenous production of H2S plays a significant role in the regulation of cellular functions, including cell growth, hyperpolarization of cell membranes, modulation of neuronal excitability and relaxation of smooth muscle cells. The CSE/H2S pathway is upregulated in the heart in a murine model of CVB3-induced myocarditis and that inhibition of endogenous H2S is beneficial to treatment early in the disease while administration of exogenous H2S is protective to infected myocardium during the later stage. Mutations in the gene encoding CTH can result in the autosomal recessive disease cystathioninuria; a disorder characterized by the unusual accumulation of plasma cystathionine causing increased urinary excretion. Both male and female CTH-null mice showed hypercystathioninemia and hyperhomocysteinemia, but not hypermethioninemia. CSE has also been reported to be expressed in endothelial cells and contribute to endothelium-dependent vasorelaxation. It can be detected a minor 36 kDa band probably representing a degradative intermediate except the major 43 kDa band in vitamin B6-deficient rat liver(PMID:8660672). CTH also can be detected as ~70kD in rat liver (PMID: 18974309). This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human CTH.

Protocols

Product Specific Protocols
WB protocol for Gamma cystathionase antibody 12217-1-APDownload protocol
IHC protocol for Gamma cystathionase antibody 12217-1-APDownload protocol
IF protocol for Gamma cystathionase antibody 12217-1-APDownload protocol
IP protocol for Gamma cystathionase antibody 12217-1-APDownload protocol
FC protocol for Gamma cystathionase antibody 12217-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Br J Pharmacol

3-mercaptopyruvate sulfurtransferase supports endothelial cell angiogenesis and bioenergetics.

Authors - Armita Abdollahi Govar
ratWB

Br J Pharmacol

Hydrogen Sulfide Regulates Muscle RING Finger-1 Protein S-Sulfhydration at Cys44 to Prevent Cardiac Structural Damage in Diabetic Cardiomyopathy.

Authors - Xiaojiao Sun
mouseWB

Nitric Oxide

Increase of cystathionine-γ-lyase (CSE) during late wound repair: Hydrogen sulfide triggers cytokeratin 10 expression in keratinocytes.

Authors - Itamar Goren
  • KO Validated
mouseWB

Free Radic Biol Med

Hydrogen sulfide protects against DSS-induced colitis by inhibiting NLRP3 inflammasome.

Authors - Ming Qin
humanWB

J Biol Chem

S-3-Carboxypropyl-L-cysteine specifically inhibits cystathionine γ-lyase-dependent hydrogen sulfide synthesis.

Authors - Pramod K Yadav
humanWB

Pharmacol Res

Histone deacetylase 6 inhibitor tubastatin A attenuates angiotensin II-induced hypertension by preventing cystathionine γ-lyase protein degradation.

Authors - Zhexi Chi

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Piruthivi (Verified Customer) (12-09-2019)

Western blot was very clear.

  • Applications: Western Blot,