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Gamma Cystathionase Polyclonal antibody
Gamma Cystathionase Polyclonal Antibody for FC, IF, IHC, IP, WB,ELISA
Cat no : 12217-1-AP
Validation Data Gallery
|Positive WB detected in||mouse kidney tissue, HepG2 cells, mouse heart tissue, rat heart tissue, HeLa cells, mouse liver tissue, rat kidney tissue, rat liver tissue|
|Positive IP detected in||mouse liver tissue|
|Positive IHC detected in||human liver cancer tissue, human breast cancer tissue, human kidney tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Positive FC detected in||MCF-7 cells|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:100-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
12217-1-AP targets Gamma Cystathionase in WB, IP, IHC, IF, FC,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, Rabbit, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Gamma Cystathionase fusion protein Ag2872|
|Full Name||cystathionase (cystathionine gamma-lyase)|
|Calculated molecular weight||405 aa, 45 kDa|
|Observed molecular weight||40-45 kDa|
|GenBank accession number||BC015807|
|Gene ID (NCBI)||1491|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
CTH, also named as Gamma-cystathionase and CSE, belongs to the transsulfuration enzymes family. It catalyzes the last step in the transsulfuration pathway from methionine to cysteine. CTH converts two cysteine molecules to lanthionine and hydrogen sulfide. CTH can also accept homocysteine as substrate. It specificity depends on the levels of the endogenous substrates. CTH is the major H2S-producing enzyme in kidney, liver, vascular smooth muscle cells and enterocytes. The endogenous production of H2S plays a significant role in the regulation of cellular functions, including cell growth, hyperpolarization of cell membranes, modulation of neuronal excitability and relaxation of smooth muscle cells. The CSE/H2S pathway is upregulated in the heart in a murine model of CVB3-induced myocarditis and that inhibition of endogenous H2S is beneficial to treatment early in the disease while administration of exogenous H2S is protective to infected myocardium during the later stage. Mutations in the gene encoding CTH can result in the autosomal recessive disease cystathioninuria; a disorder characterized by the unusual accumulation of plasma cystathionine causing increased urinary excretion. Both male and female CTH-null mice showed hypercystathioninemia and hyperhomocysteinemia, but not hypermethioninemia. CSE has also been reported to be expressed in endothelial cells and contribute to endothelium-dependent vasorelaxation. It can be detected a minor 36 kDa band probably representing a degradative intermediate except the major 43 kDa band in vitamin B6-deficient rat liver(PMID:8660672). CTH also can be detected as ~70kD in rat liver (PMID: 18974309). This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human CTH.
|Product Specific Protocols|
|WB protocol for Gamma Cystathionase antibody 12217-1-AP||Download protocol|
|IHC protocol for Gamma Cystathionase antibody 12217-1-AP||Download protocol|
|IF protocol for Gamma Cystathionase antibody 12217-1-AP||Download protocol|
|IP protocol for Gamma Cystathionase antibody 12217-1-AP||Download protocol|
|FC protocol for Gamma Cystathionase antibody 12217-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Br J Pharmacol
3-mercaptopyruvate sulfurtransferase supports endothelial cell angiogenesis and bioenergetics.
Br J Pharmacol
Hydrogen Sulfide Regulates Muscle RING Finger-1 Protein S-Sulfhydration at Cys44 to Prevent Cardiac Structural Damage in Diabetic Cardiomyopathy.
Increase of cystathionine-γ-lyase (CSE) during late wound repair: Hydrogen sulfide triggers cytokeratin 10 expression in keratinocytes.
Free Radic Biol Med
Hydrogen sulfide protects against DSS-induced colitis by inhibiting NLRP3 inflammasome.
J Biol Chem
S-3-Carboxypropyl-L-cysteine specifically inhibits cystathionine γ-lyase-dependent hydrogen sulfide synthesis.
Histone deacetylase 6 inhibitor tubastatin A attenuates angiotensin II-induced hypertension by preventing cystathionine γ-lyase protein degradation.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Piruthivi (Verified Customer) (12-09-2019)
Western blot was very clear.