CoraLite®594-conjugated CUL4A Monoclonal antibody

CUL4A Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

human, monkey, mouse, rat, pig

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1A7F12

Cat no : CL594-66038

Synonyms

CUL 4A, CUL4A, cullin 4A



Tested Applications

Positive IF detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 66038-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of CL594-66038 is CUL4A Fusion Protein expressed in E. coli.

Tested Reactivity human, monkey, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CUL4A fusion protein Ag18089
Full Name cullin 4A
Calculated molecular weight 77 kDa
Observed molecular weight 77 kDa, 88 kDa
GenBank accession numberBC008308
Gene symbol CUL4A
Gene ID (NCBI) 8451
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths593 nm/614 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cullin proteins assemble a large number of RING E3 ubiquitin ligases, participating in the proteolysis through the ubiquitin-proteasome pathway. Two cullin 4 (CUL4) proteins, CUL4A (87 kDa) and CUL4B(104 kDa), have been identified. The two CUL4 sequences are 83% identical. They target certain proteins for degradation by binding protein DDB1 to form a CUL4-DDB1 ubiquitin ligase complex with DDB. They form two individual E3 ligases, DDB1-CUL4ADDB2 and DDB1-CUL4BDDB2 in this process. CUL4A appeared in both the nucleus and the cytosol, suggesting a more complex mechanism for entering the nucleus. CUL4B is localized in the nucleus and facilitates the transfer of DDB1 into the nucleus independently of DDB2.

Protocols

Product Specific Protocols
IF protocol for CL594 CUL4A antibody CL594-66038Download protocol
Standard Protocols
Click here to view our Standard Protocols