Tested Applications
| Positive WB detected in | TT cells, ATDC-5 cells |
| Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human lung cancer tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
86139-2-RR targets Collagen Type II in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag29265 Product name: Recombinant human COL2A1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-63 aa of BC007252 Sequence: MSAFAGLGPREKGPDPLQYMRADQAAGGLRQHDAEVDATLKSLNNQIESIRSPEGSRKNPART Predict reactive species |
| Full Name | collagen, type II, alpha 1 |
| Calculated Molecular Weight | 134 kDa |
| Observed Molecular Weight | 200 kDa |
| GenBank Accession Number | BC007252 |
| Gene Symbol | COL2A1 |
| Gene ID (NCBI) | 1280 |
| RRID | AB_3744654 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P02458 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The COL2A1 gene encodes the alpha-1 chain of type II collagen, a fibrillar collagen found primarily in cartilage and the vitreous humor of the eye. Type II collagen is essential for the formation of the extracellular matrix in cartilage and other connective tissues, providing structure and strength to these tissues.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Collagen Type II antibody 86139-2-RR | Download protocol |
| IHC protocol for Collagen Type II antibody 86139-2-RR | Download protocol |
| WB protocol for Collagen Type II antibody 86139-2-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Collagen Type II Recombinant monoclonal antibody (250506B7), catalog number 86139-2-RR, has 16 citations. These appear in journals including Bioactive Materials, Carbohydrate Polymers, Advanced Healthcare Materials, Journal of Ethnopharmacology, Life Sciences, Bone & Joint Research, Biological Direct, Chemical Biology & Interaction, Journal of Orthopaedic Surgery and Research, Tissue & Cell, Acta Histochemica, Journal of Molecular Histology, Parasite Immunology, Cell Biochemistry and Biophysics, and Experimental Molecular Medicine. Research fields center on osteoarthritis, cartilage degeneration, intervertebral disc degeneration, rheumatoid arthritis, chondrocyte apoptosis/pyroptosis, and macrophage polarization.
| Species | Application | Title |
|---|---|---|
Bioact Mater A nature-derived, strong adhesive hydrogel microsphere for wet tissue repair: Multifunctional and clinical potential. | ||
Carbohydr Polym Ultrasound-triggered injectable piezoelectric nanocomposite hyaluronic acid-based hydrogel for modulating inflammation and chondrogenesis in osteoarthritis treatment. | ||
Adv Healthc Mater Remodeling Cellular Uptake via Inducing Clustering of Urokinase Receptors in Hydrogel Microspheres for Alleviating Cartilage Senescence. | ||
J Ethnopharmacol Integrated prioritization of candidate quality markers for Smilacis Chinae Rhizoma under the five-principle framework through chemical profiling, effectiveness evaluation, and bionic sensing. | ||
Life Sci Compressive stress induces cartilage endplate degeneration through the Piezo1/YAP-TEAD/NLRP3 axis.
| ||
Biol Direct Programmed cell death-related gene S100A9 promotes macrophage M1 polarization and chondrocyte apoptosis in rheumatoid arthritis. |
