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Collagen Type II Recombinant monoclonal antibody, PBS Only (86139-2-PBS)

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WB analysis using 86139-2-RR (same clone as 86139-2-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 86139-2-RR (Collagen Type II antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86139-2-PBS in a different storage buffer formulation.
WB analysis of TT using 86139-2-RR (same clone as 86139-2-PBS)
TT cells were subjected to SDS PAGE followed by western blot with 86139-2-RR (COL2A1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86139-2-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 86139-2-RR (same clone as 86139-2-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 86139-2-RR (Collagen Type II antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86139-2-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 86139-2-RR (same clone as 86139-2-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 86139-2-RR (Collagen Type II antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86139-2-PBS in a different storage buffer formulation.
Affinity and Kinetic Characterization of 86139-2-RR
Biolayer interferometry (BLl) kinetic assays of 86139-2-RR against Human Collagen Type II were performed. The affinity constant is 4.96 nM.

Collagen Type II Recombinant monoclonal antibody, PBS Only

Name: Collagen Type II Recombinant monoclonal antibody, PBS Only
Brand: Proteintech
SKU: 86139-2-PBS

Collagen Type II Uni-rAb^® Recombinant Antibody for WB, IHC, Indirect ELISA

Cat No. 86139-2-PBS

Clone No.250506B7

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

COL2, COL2A1, Alpha 1 type II collagen, Alpha-1 type II collagen, ANFH

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

86139-2-PBS targets Collagen Type II in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Ag29265 Product name: Recombinant human COL2A1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-63 aa of BC007252 Sequence: MSAFAGLGPREKGPDPLQYMRADQAAGGLRQHDAEVDATLKSLNNQIESIRSPEGSRKNPART Predict reactive species
Full Name	collagen, type II, alpha 1
Calculated Molecular Weight	134 kDa
Observed Molecular Weight	200 kDa
GenBank Accession Number	BC007252
Gene Symbol	COL2A1
Gene ID (NCBI)	1280
RRID	AB_3744654
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P02458
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

The COL2A1 gene encodes the alpha-1 chain of type II collagen, a fibrillar collagen found primarily in cartilage and the vitreous humor of the eye. Type II collagen is essential for the formation of the extracellular matrix in cartilage and other connective tissues, providing structure and strength to these tissues.