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Cytochrome c Antibody
Cytochrome c Monoclonal Antibody for IF, IHC, WB, ELISA
Cat no : 66264-1-Ig
CYC, CYCS, Cytochrome c, cytochrome c, somatic, HCS, THC4
|Positive WB detected in||HeLa cells, human heart tissue, human skeletal muscle tissue, rat skeletal muscle tissue, mouse skeletal muscle tissue, HEK-293 cells|
|Positive IHC detected in||human liver cancer tissue, human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunohistochemistry (IHC)||IHC : 1:1000-1:5000|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
66264-1-Ig targets Cytochrome c in IF, IHC, WB, ELISA applications and shows reactivity with human, mouse, rat samples.
|Reactivity||human, mouse, rat|
|Cited Species||human, mouse, rat|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Cytochrome c fusion protein Ag24349|
|Full Name||cytochrome c, somatic|
|Calculated molecular weight||12 kDa|
|Observed molecular weight||12-15 kDa|
|GenBank accession number||BC009578|
|Gene ID (NCBI)||54205|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20oC. Aliquoting is unnecessary for -20oC storage.|
Cytochrome c is a 12-15 kDa electron transporting protein located in the inner mitochondrial membrane. Upon apoptotic stimulation, cytochrome c can be released from mitochondria into cytoplasm, resulting in caspase-3 activation and apoptosis. Measurement of cytochrome c release from the mitochondria is useful for detection of the onset of apoptosis in cells. In addition, cytochrome c can also leave cells and be detectable in extra-cellular medium of apoptotic cells and serum of cancer patients. The level of serum cytochrome c may serve as a prognostic maker during cancer therapy.
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Megha Singhal (07-10-2018)