Tested Applications
| Positive WB detected in | human saliva |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
84042-1-RR targets Cytokeratin 3 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | keratin 3 |
| Calculated Molecular Weight | 64 kDa |
| Observed Molecular Weight | 64 kDa |
| GenBank Accession Number | NM_057088 |
| Gene Symbol | Cytokeratin 3 |
| Gene ID (NCBI) | 3850 |
| RRID | AB_3671609 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P12035 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. As a member of the type II cytokeratin subfamily, KRT3 is expressed together with KRT12 during 'corneal-type' differentiation.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Cytokeratin 3 antibody 84042-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Cytokeratin 3 (KRT3) recombinant monoclonal antibody (catalog number 84042-1-RR) has 1 total citation. It appears in Investigative Ophthalmology and Visual Science (2025), in a study on keratin expression in normal and pathological corneas and Krt17's regulatory role on limbal stem cells. The associated research field is ophthalmology/ocular surface biology, with the antibody applied in Western blot for human samples.
| Species | Application | Title |
|---|---|---|
Invest Ophthalmol Vis Sci Expression Distribution of Keratins in Normal and Pathological Corneas and the Regulatory Role of Krt17 on Limbal Stem Cells |
