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DEPDC6 Polyclonal antibody

DEPDC6 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21312-1-AP

Synonyms

DEP domain containing 6, DEP.6, DEPDC6, Deptor

Validation Data Gallery

  • WB analysis of HeLa using 21312-1-AP

    WB analysis of HeLa using 21312-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 21312-1-AP (DEPDC6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human thyroid cancer using 21312-1-AP

    IHC staining of human thyroid cancer using 21312-1-AP

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer slide using 21312-1-AP (DEPDC6 Antibody) at dilution of 1:50.

Tested Applications

Positive WB detected inHeLa cells, COLO 320 cells, HT-1080 cells
Positive IHC detected inhuman thyroid cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 21312-1-AP is DEPDC6 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DEPDC6 fusion protein Ag15959
Full Name DEP domain containing 6
Calculated molecular weight 409 aa, 46 kDa
Observed molecular weight 55 kDa
GenBank accession numberBC024746
Gene symbol DEPDC6
Gene ID (NCBI) 64798
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DEPTOR, also named as DEPDC6, is a negative regulator of the mTORC1 and mTORC2 signaling pathways. Through mTOR interactions, DEPTOR is implicated in pathways for cell growth and differentiation, as well as cell-cycle prolongation or termination. It inhibits the kinase activity of both complexes. The MW will migrate to 50-55 kDa with phospho- form.

Protocols

Product Specific Protocols
WB protocol for DEPDC6 antibody 21312-1-APDownload protocol
IHC protocol for DEPDC6 antibody 21312-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols