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DEPDC6 Polyclonal antibody

DEPDC6 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 21312-1-AP

Synonyms

DEP domain containing 6, DEP.6, DEPDC6, Deptor

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using DEPDC6 Polyclonal antibody (21312-1-AP)

    WB analysis of HeLa using 21312-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 21312-1-AP (DEPDC6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HT-1080 cells using DEPDC6 Polyclonal antibody (21312-1-AP)

    WB analysis of HT-1080 using 21312-1-AP

    HT-1080 cells were subjected to SDS PAGE followed by western blot with 21312-1-AP (DEPDC6 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using DEPDC6 Polyclonal antibody (21312-1-AP)

    WB analysis of COLO 320 using 21312-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 21312-1-AP (DEPDC6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using DEPDC6 Polyclonal antibody (21312-1-AP)

    IHC staining of human thyroid cancer using 21312-1-AP

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer slide using 21312-1-AP (DEPDC6 Antibody) at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using DEPDC6 Polyclonal antibody (21312-1-AP)

    IHC staining of human thyroid cancer using 21312-1-AP

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer slide using 21312-1-AP (DEPDC6 Antibody) at dilution of 1:50.

Tested Applications

Positive WB detected inHeLa cells, COLO 320 cells, HT-1080 cells
Positive IHC detected inhuman thyroid cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 21312-1-AP is DEPDC6 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DEPDC6 fusion protein Ag15959
Full Name DEP domain containing 6
Calculated Molecular Weight 409 aa, 46 kDa
Observed Molecular Weight 55 kDa
GenBank Accession NumberBC024746
Gene Symbol DEPDC6
Gene ID (NCBI) 64798
RRIDAB_2878837
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DEPTOR, also named as DEPDC6, is a negative regulator of the mTORC1 and mTORC2 signaling pathways. Through mTOR interactions, DEPTOR is implicated in pathways for cell growth and differentiation, as well as cell-cycle prolongation or termination. It inhibits the kinase activity of both complexes. The MW will migrate to 50-55 kDa with phospho- form.

Protocols

Product Specific Protocols
WB protocol for DEPDC6 antibody 21312-1-APDownload protocol
IHC protocol for DEPDC6 antibody 21312-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols