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DEPDC6 Antibody 0 Publications

Rabbit Polyclonal| Catalog number: 21312-1-AP

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Con: 31 μg/150 μl

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Tested Applications

Applications:
WB, IHC, ELISA
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Images See all

DEPDC6 Antibody WB HeLa cells 21312-1-AP

HeLa cells were subjected to SDS PAGE followed by western blot with 21312-1-AP(DEPDC6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

DEPDC6 Antibody IHC human thyroid cancer tissue 21312-1-AP

Immunohistochemistry of paraffin-embedded human thyroid cancer slide using 21312-1-AP( DEPDC6 Antibody) at dilution of 1:50

Species specificity:
human
Positive WB detected in:
HeLa cells, COLO 320 cells, HT-1080 cells
Positive IHC detected in:
human thyroid cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Recommended dilution:
WB : 1:500-1:1000
IHC : 1:20-1:200

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
DEPDC6 fusion protein Ag15959
Full name:
DEP domain containing 6
Calculated molecular weight:
409aa,46 kDa
Observed molecular weight:
55 kDa
GenBank accession number:
BC024746
Gene ID (NCBI):
64798
Gene symbol
DEPDC6
Synonyms
DEP domain containing 6, DEP.6, DEPDC6, Deptor

Background

DEPTOR, also named as DEPDC6, is a negative regulator of the mTORC1 and mTORC2 signaling pathways. Through mTOR interactions, DEPTOR is implicated in pathways for cell growth and differentiation, as well as cell-cycle prolongation or termination. It inhibits the kinase activity of both complexes. The MW will migrate to 50-55 kDa with phospho- form.


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