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DLX3 Polyclonal antibody

DLX3 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 13261-3-AP

Synonyms

AI4, distal less homeobox 3, DLX3, Homeobox protein DLX 3, TDO

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DLX3 Polyclonal antibody (13261-3-AP)

    WB analysis using 13261-3-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13261-3-AP (DLX3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse lung tissue using DLX3 Polyclonal antibody (13261-3-AP)

    WB analysis of mouse lung using 13261-3-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 13261-3-AP (DLX3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using DLX3 Polyclonal antibody (13261-3-AP)

    IHC staining of human lung cancer using 13261-3-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 13261-3-AP (DLX3 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using DLX3 Polyclonal antibody (13261-3-AP)

    IHC staining of human lung cancer using 13261-3-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 13261-3-AP (DLX3 antibody) at dilution of 1:100 (under 10x lens).

Tested Applications

Positive WB detected inJAR cells, mouse lung tissue, NCCIT cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13261-3-AP targets DLX3 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DLX3 fusion protein Ag3865
Full Name distal-less homeobox 3
Calculated Molecular Weight 287 aa, 32 kDa
Observed Molecular Weight 32-38 kDa
GenBank Accession NumberBC028970
Gene Symbol DLX3
Gene ID (NCBI) 1747
RRIDAB_2292996
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Distal-less homeobox 3 (DLX3), a member of the Dlx family, plays a crucial role in epithelium, hair, bone, tooth and placental development. DLX3 is expressed during the calcium (Ca2+)-dependent epidermal differentiation process. The calculated molecular weight of DLX3 is 32 kDa. This antibody detects the 32-38 kDa protein in SDS-PAGE. (PMID: 31029881)

Protocols

Product Specific Protocols
WB protocol for DLX3 antibody 13261-3-APDownload protocol
IHC protocol for DLX3 antibody 13261-3-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IP,CoIP,IF

J Biol Chem

New insights into the regulation of placental growth factor gene expression by the transcription factors GCM1 and DLX3 in human placenta.

Authors - Yueh-Ho Chiu
View Article
mouseWB,CoIP

Stem Cells

IPO7 promotes odontoblastic differentiation and inhibits osteoblastic differentiation through regulation of RUNX2 expression and translocation

Authors - Yue Zhang
View Article