DOCK9 Polyclonal antibody

DOCK9 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 18987-1-AP

Synonyms

dedicator of cytokinesis 9, DOCK9, KIAA1058, KIAA1085, ZIZ1, ZIZIMIN1



Tested Applications

Positive WB detected inA549 cells, HeLa cells, human brain tissue, human heart tissue, human placenta tissue, MCF-7 cells, mouse brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman kidney tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

18987-1-AP targets DOCK9 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name dedicator of cytokinesis 9
Calculated molecular weight 239 kDa
Observed molecular weight 200-236 kDa
GenBank accession numberNM_015296
Gene symbol DOCK9
Gene ID (NCBI) 23348
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DOCK9 is a guanine nucleotide exchange factor (GEF) that activates CDC42 by exchanging bound GDP for free GTP. Its overexpression induces filopodia formation.

Protocols

Product Specific Protocols
WB protocol for DOCK9 antibody 18987-1-APDownload protocol
IHC protocol for DOCK9 antibody 18987-1-APDownload protocol
IF protocol for DOCK9 antibody 18987-1-APDownload protocol
IP protocol for DOCK9 antibody 18987-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols