DRD1 Polyclonal antibody

Name: DRD1 Rabbit Polyclonal antibody
Brand: Proteintech
SKU: 17934-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 17934-1-AP

Synonyms

D(1A) dopamine receptor, DADR, Dopamine D1 receptor, dopamine receptor D1, DRD1, DRD1A

WB analysis of mouse brain using 17934-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 17934-1-AP (DRD1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IHC staining of mouse brain using 17934-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17934-1-AP (DRD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 17934-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17934-1-AP (DRD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse brain using 17934-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using DRD1 antibody (17934-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of mouse brain using 17934-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using DRD1 antibody (17934-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Cat No. 17934-1-AP

Freight/Packing: -

Country/Region

Size/Concentration

Quantity

Positive WB detected in	mouse brain tissue, SH-SY5Y cells
Positive IHC detected in	mouse brain tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF detected in	mouse brain tissue

Application	Dilution
Western Blot (WB)	WB : 1:200-1:1000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)	IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

KD/KO	See 1 publications below
WB	See 3 publications below

17934-1-AP targets DRD1 in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	DRD1 fusion protein Ag12366
Full Name	dopamine receptor D1
Calculated molecular weight	446 aa, 49 kDa
Observed molecular weight	50 kDa
GenBank accession number	BC074978
Gene symbol	DRD1
Gene ID (NCBI)	1812
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Product Specific Protocols
WB protocol for DRD1 antibody 17934-1-AP	Download protocol
IHC protocol for DRD1 antibody 17934-1-AP	Download protocol
IF protocol for DRD1 antibody 17934-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Species	Application	Title
human	WB	Mol Neurobiol Protective Effect of Curcumin by Modulating BDNF/DARPP32/CREB in Arsenic-Induced Alterations in Dopaminergic Signaling in Rat Corpus Striatum. Authors - Pranay Srivastava View Article
rat	WB	EMBO Mol Med MicroRNA expression profile and functional analysis reveal that miR-382 is a critical novel gene of alcohol addiction. Authors - Li Jingyuan J View Article
human	WB	Cell PMID: 25594175 Authors - Yiqing Yan KD Validated View Article